首页 | 本学科首页   官方微博 | 高级检索  
检索        

Determination of the Early Time of Death by Computerized Image Analysis of DNA Degradation: Which Is the Best Quantitative Indicator of DNA Degradation?
引用本文:刘丽江,舒细记,任亮,周红艳,李艳,柳威,朱丞,刘良.Determination of the Early Time of Death by Computerized Image Analysis of DNA Degradation: Which Is the Best Quantitative Indicator of DNA Degradation?[J].华中科技大学学报(医学英德文版),2007,27(4):362-366.
作者姓名:刘丽江  舒细记  任亮  周红艳  李艳  柳威  朱丞  刘良
作者单位:[1]Faculty of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China [2]School of Medicine & Life Sciences, Jianghan University, Wuhan 430056, China
基金项目:The project was supported by a grant form the Wuhan Municipal Chengguang Research Program (No, 20015005049).
摘    要:This study evaluated the correlation between DNA degradation of the splenic lympho-cytes and the early time of death,examined the early time of death by computerized image analysis technique(CIAT) and identified the best parameter that quantitatively reflects the DNA degradation.The spleen tissues from 34 SD rats were collected,subjected to cell smearing every 2 h within the first 36 h after death,stained by Feulgen-Van's staining,three indices reflecting DNA content in splenic lymphocytes,including integral optical density(IOD),average optical density(AOD),aver-age gray scale(AG) were measured by the image analysis.Our results showed that IOD and AOD decreased and AG increased over time within the first 36 h.A stepwise linear regression analysis showed that only AG was fitted.A correlation between the postmortem interval(PMI) and AG was identified and the corresponding regression equation was obtained.Our study suggests that CIAT is a useful and promising tool for the estimation of early PMI with good objectivity and reproducibility,and AG is a more effective and better quantitative indicator for the estimation of PMI within the first 36 h after death in rats.

关 键 词:图象分析  DNA降解  法医病理学  尸检
收稿时间:20 March 2007
修稿时间:2007-03-20

Determination of the early time of death by computerized image analysis of DNA degradation: Which is the best quantitative indicator of DNA degradation?
Liu?Lijiang,Shu?Xiji,Ren?Liang,Zhou?Hongyan,Li?Yan,Liu?Wei,Zhu?Cheng,Liu?Liang.Determination of the early time of death by computerized image analysis of DNA degradation: Which is the best quantitative indicator of DNA degradation?[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2007,27(4):362-366.
Authors:Liu Lijiang  Shu Xiji  Ren Liang  Zhou Hongyan  Li Yan  Liu Wei  Zhu Cheng  Liu Liang
Institution:1. Faculty of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
2. Faculty of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China;School of Medicine & Life Sciences, Jianghan University, Wuhan 430056, China
3. School of Medicine & Life Sciences, Jianghan University, Wuhan 430056, China
Abstract:This study evaluated the correlation between DNA degradation of the splenic lympho-cytes and the early time of death,examined the early time of death by computerized image analysis technique(CIAT) and identified the best parameter that quantitatively reflects the DNA degradation.The spleen tissues from 34 SD rats were collected,subjected to cell smearing every 2 h within the first 36 h after death,stained by Feulgen-Van's staining,three indices reflecting DNA content in splenic lymphocytes,including integral optical density(IOD),average optical density(AOD),aver-age gray scale(AG) were measured by the image analysis.Our results showed that IOD and AOD decreased and AG increased over time within the first 36 h.A stepwise linear regression analysis showed that only AG was fitted.A correlation between the postmortem interval(PMI) and AG was identified and the corresponding regression equation was obtained.Our study suggests that CIAT is a useful and promising tool for the estimation of early PMI with good objectivity and reproducibility,and AG is a more effective and better quantitative indicator for the estimation of PMI within the first 36 h after death in rats.
Keywords:forensic pathology  postmortem interval  DNA degradation  image analysis
本文献已被 维普 万方数据 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号