| NisP Is Related to Nisin Precursor Processing and Possibly to Immunity in Lactococcus Lactis |
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| 引用本文： | 叶嗣颖.NisP Is Related to Nisin Precursor Processing and Possibly to Immunity in Lactococcus Lactis[J].华中科技大学学报(医学英德文版),1995,15(4):193-197. |
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| 作者姓名： | 叶嗣颖 |
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| 作者单位： | YE Si-ying;Koponen O2; Qiao M2，Immonen T2; Saris P E J2(Department of Microbiology，Tongji Medical University. Wuhan 430030)(Institute of Biotechnolegy，Valimotie 7，P. O. Box 45，University of Helsinki，Finland，SF-00014) |
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| 摘 要： | ＮｉｓＰＩｓＲｅｌａｔｅｄｔｏＮｉｓｉｎＰｒｅｃｕｒｓｏｒＰｒｏｃｅｓｓｉｎｇａｎｄＰｏｓｓｉｂｌｙｔｏＩｍｍｕｎｉｔｙｉｎＬａｃｔｏｃｏｃｃｕｓＬａｃｔｉｓＹＥＳｉ－ｙｉｎｇ（叶嗣颖）；ＫｏｐｏｎｅｎＯ２；ＱｉａｏＭ２，ＩｍｍｏｎｅｎＴ２；Ｓａｒｉ...
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| 收稿时间： | 28 March 1995 |
NisP is related to nisin precursor processing and possibly to immunity in lactococcus lactis |
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| Ye Si-ying,O. Koponen,M. Qiao,T. Immonen,P. E. J. Saris.NisP is related to nisin precursor processing and possibly to immunity in lactococcus lactis[J].Journal of Zuazhong University of Science and Technology: Medical Edition,1995,15(4):193-197. |
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| Authors: | Ye Si-ying O Koponen M Qiao T Immonen P E J Saris |
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| Institution: | (1) Department of Microbiology, Tongji Medical University, 430030 Wuhan;(2) Institute of Biotechnology, University of Helsinki, Valimotie 7, P.O. Box 45, SF-00014, Finland |
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| Abstract: | In this study, a plasmid was integrated into nisP, creating the first defined mutation in a nisin biosynthetic gene. The mutant strain secreted fully modified nisin with the N-terminal leader still attached.The presence of the leader was confirmed by N-terminal sequencing of the purified precursor. The dehydration and lanthionine formation of the precursor were already completed as active nisin could be formed by cleaving the leader from the inactive precursor by a trypsin treatment or by incubation with wild type cells. Nisin immunity of the NisP mutant strain was lowered to about 10% of the wild type immunity. The results show that NisP is needed for precursor processing and for development of high immunity of nisin. |
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| Keywords: | Nisin Lactococcus lactis nisin immunity gene mutation |
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