| Cloning and Expression of Rat Transforming Growth Factor β1 cDNA in Osteoblasts |
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| 引用本文： | 刘勇,郑启新,杜靖远,曾晖,郭晓东,屈伸.Cloning and Expression of Rat Transforming Growth Factor β1 cDNA in Osteoblasts[J].华中科技大学学报(医学英德文版),2000,20(1). |
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| 作者姓名： | 刘勇 郑启新 杜靖远 曾晖 郭晓东 屈伸 |
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| 作者单位： | LIU Yong，ZHENG Qixin，DU Jingyuan，ZENG Hui，GUO Xiaodong (Department of Orthopaedics，Xiehe Hospital，Tongji Medical University，Wuhan 430022 |
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| 摘 要： | In order to study transforming growth factor81 (TGF51) in molecular level and investigate thefeasibility of TGF51 gene therapy for bone defects,an eukaryotic expression vector containing ratTGF51 cDNA was constructed. The expression ofTGF51 in the transfected osteoblasts was observed.1 MATERIALS AND METHODS1. 1 Animals and Cell CultureSprague--Dawley rats, I adult and 5 newborn,were purchased from Animal Center of Tong nMedical University. Lymphocytes, isolated fromperipheral…
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| 关 键 词： | transforming growth factor β1 gene expression RT-PCR osteoblasts molecular cloning |
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