Correlation between physical status of human papilloma virus and cervical carcinogenesis |
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Authors: | Kezhen Li Xin Jin Yong Fang Changyu Wang Mei Gong Pingbo Chen Jia Liu Dongrui Deng Jihui Ai |
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Institution: | Cancer Biology Research Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. tjkeke@126.com |
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Abstract: | The prevalence of human papilloma virus (HPV)-16 in patients with cervical cancer, the physical status of HPV-16 in patients
with cervical lesions, and the role of HPV-16 integration in cervical carcinogenesis were investigated. HPV genotyping was
performed by using PCR approach with the primer GP5+/GP6+ and type-specific primer on biopsy specimens taken operatively from
198 women. Multiple PCR was done to detect physical status of HPV-16 in a series of cervical liquid-based cytology samples
and biopsy specimens obtained from different cervical lesions with HPV-16 infection, including 112 specimens with cervical
cancer, 151 specimens with CIN I, 246 specimens with CIN and 120 specimens with CINIII. The results showed that there were
112 cervical cancer samples (56.57% of total cervical cancer patients) with HPV-16 infection. The frequency of HPV-16 pure
integration was 65.18% (73/112), 56.57% (47/120), 23.58% (58/246) and 7.95% (12/151) in cervical cancer, CINIII, CINII and
CINI patients respectively. In situ hybridization was performed on some paraffin-embedded sections of CINII, CINIII and cervical cancer to verify the physical
status of HPV-16 infection. Significant difference was observed between cervical cancer and CIN I, CINII, CINIII in the frequency
of HPV-16 integration (P<0.01). It is suggested that HPV-16 is the most prevalent type and is associated with cervical cancer. In the case of HPV-16
infection there are close associations between the severity of cervical lesions and the frequency of HPV-16 integration. The
application of testing HPV genotyping and physical status based on detection of HC-II HPV DNA would be in favor of predicting
the prognosis of cervical precancerosis and enhancing the screening accuracy of cervical cancer. |
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Keywords: | human papilloma virus cervical cancer genotyping physical status integration |
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