| An Experimental Study on the Regulation of Expression of Th_2 Cytokines from T Lymphocytes by Protein Kinase C in Asthma |
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| 作者姓名： | 熊维宁 徐永健 张珍祥 王孝养 莫碧文 傅娟 |
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| 作者单位： | XIONG Weining，XU Yongjian，ZHANG Zhenxiang，WANG Xiaoyang，MO Biwen，FU Juan Department of Respiratory Medicine，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430030 |
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| 基金项目： | This project was supported by a grant from National
Natural Sciences foundation (No.30 0 70 332 ) and Education
Ministry college cadreman teachers imbursement plan (2 0 0 0
year) |
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| 摘 要： | Bronchial asthma (asthma) was a disease ofchronic airway inflammation.Inflammatory cells,primarily eosinophils and lymphocytes,andinflammatory proteins including cytokines generatedby them participated in pathogenesis of asthma.Interleukin- 4(IL- 4) and IL- 5 ,primarily generated bytype helper T lymphocytes(Th2 ) ,were consideredto be pivotal inflammation- promoting cytokines atpresent,and their expression was up- regulated inasthmatic process1] ,but the idiographic regulatorymechanisms wer…
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An Experimental Study on the Regulation of Expression of Th_2 Cytokines from T Lymphocytes by Protein Kinase C in Asthma |
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| XIONG Weining,XU Yongjian,ZHANG Zhenxiang,WANG Xiaoyang,MO Biwen,FU Juan.An Experimental Study on the Regulation of Expression of Th_2 Cytokines from T Lymphocytes by Protein Kinase C in Asthma[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2001(4). |
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| Authors: | XIONG Weining XU Yongjian ZHANG Zhenxiang WANG Xiaoyang MO Biwen FU Juan |
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| Institution: | XIONG Weining,XU Yongjian,ZHANG Zhenxiang,WANG Xiaoyang,MO Biwen,FU Juan Department of Respiratory Medicine,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030 |
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| Abstract: | To explore the regulatory role of protein kinase C (PKC) in the expression of Th 2 cytokines, interleukin 4 (IL 4) and interleukin 5 (IL 5) by T lymphocytes in asthma. T lymphocytes were isolated and purified from blood and bronchial alveolus lavage fluid (BALF) of each guinea pig of normal control group and asthmatic group and from peripheral blood of the asthmatic patients and normal controls, and were stimulated with PKC accelerant phorbol 12 myristate 13 acetate (PMA) and inhibitor Ro31 8220. The expression of IL 4 and IL 5 mRNA and protein was detected by using in situ hybridization staining and ELISA respectively. The expression of IL 4 and IL 5 mRNA and protein of asthmatic T lymphocytes stimulated with PMA was significantly higher than that of asthmatic T lymphocytes stimulated without PMA respectively ( P <0.01) and that of normal T lymphocytes stimulated with PMA respectively ( P <0.01). The expression of IL 4 and IL 5 mRNA and protein of asthmatic T lymphocytes stimulated with PMA and Ro31 8220 was significantly lower than that of asthmatic T lymphocytes stimulated only with PMA respectively ( P <0.01). It was concluded that PKC might participate in regulating the expression of IL 4 and IL 5 in asthmatic T lymphocytes, and the activation of PKC in T lymphocytes might play an important role in the pathogenesis of asthma. |
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| Keywords: | protein kinase C bronchial asthma interleukin 4 interleukin 5 |
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