| Construction of Shuttle Expression Plasmid and Stable Expression of Foreign Gene in Mycobacteria and E．Coli |
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| 引用本文： | 皇甫永修,张大军,程继忠,钱明,梁驹卿,李东.Construction of Shuttle Expression Plasmid and Stable Expression of Foreign Gene in Mycobacteria and E．Coli[J].华中科技大学学报(医学英德文版),1995(3). |
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| 作者姓名： | 皇甫永修 张大军 程继忠 钱明 梁驹卿 李东 |
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| 作者单位： | Department of Medical Molecular Biology，Research Center of Experimental Medicine，Tongji Medical University，Wuhan 430030 |
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| 摘 要： | ＣｏｎｓｔｒｕｃｔｉｏｎｏｆＳｈｕｔｔｌｅＥｘｐｒｅｓｓｉｏｎＰｌａｓｍｉｄａｎｄＳｔａｂｌｅＥｘｐｒｅｓｓｉｏｎｏｆ ＦｏｒｅｉｇｎＧｅｎｅｉｎＭｙｃｏｂａｃｔｅｒｉａａｎｄＥ．ＣｏｌｉＨＵＡＮＧＦＵＹｏｎｇ－ｍｕ（皇甫永修）；ＺＨＡＮＧＤａ－ｊｕ...
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Construction of Shuttle Expression Plasmid and Stable Expression of Foreign Gene in Mycobacteria and E.Coli |
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| HUANGFU Yong-mu, ZHANG Da-jun, CHENG Ji-zhong,QIAN Min, LIANG Ju-qing,LI Dong.Construction of Shuttle Expression Plasmid and Stable Expression of Foreign Gene in Mycobacteria and E.Coli[J].Journal of Zuazhong University of Science and Technology: Medical Edition,1995(3). |
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| Authors: | HUANGFU Yong-mu ZHANG Da-jun CHENG Ji-zhong QIAN Min LIANG Ju-qing LI Dong |
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| Abstract: | By employing the pUC19 as a backbone,the regulatory and signal sequences which encode kanamycin resistance, and mycobacterial plasmid origin of replication (oriM) were cloned into the pUC19. The recombinant E. Coli-mycobacteria shuttle expression plasmid PBCG-8000 was constructed. The PBCG-8000 was able to replicate in both E. Coli and mycobacteria (including BCG) systems, and to confer stable kanamycin resistance upon transformants. The study should facilitate the development of BCG and other mycobacteria into multivalent vaccine vectors. |
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| Keywords: | mycobacteria shuttle plasmid expression vector BCG vector foreign gene expression |
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