Effects of cyclosporine A on angiotensin II-induced cardiomyocyte hypertrophy in neonatal rats |
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Authors: | Han Zhaomin Yang Yunhai Zhang Kailun and Xiang Jizhou |
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Institution: | (1) Department of Pharmacy, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430022 Wuhan, China;(2) Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430022 Wuhan, China;(3) Department of Pharmacology, School of Basic Medical Sciences, Tongji Medical College, Huazhong University of Science and Technology, 430022 Wuhan, China |
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Abstract: | Summary The effects of cyclosporine A (CsA) on Angiontensin II (Ang II)-induced protein contents, c-fos protein levels and cytosolic
Ca2+ level (Ca2+]i) in cultured cardiomyocytes of neonatal rats were observed. Total protein contents were determined by Bradford method.
The expression of c-fos protein was detected by Western blot. Ca2+]i labeled with fluorescent probe Fluo-3/AM was measured under a laser scanning confocal microscope. The results revealed
that as compared with control, the total protein contents were increased in cardiomyocytes treated with Ang II (10−7 mol/ L), which could be inhibited by CsA in a dose-dependent manner. It was found that Ang II could increase the c-fos protein
expression, which could be inhibited by CsA in a dose-dependent manner. Ang II induced the Ca2+]i elevation in cardiomyocytes. CsA did not influence the resting intracellular Ca2+, but inhibited significantly the Ang II-induced Ca2+]i elevation. It was concluded that CsA can suppress the Ang II-induced c-fos protein expression and Ca2+]i elevation in single cardiomyocyte, which might pay a role in the prevention of Ang II-induced cardiomyocyte hypertrophy
by CsA.
Han Zhaomin, female, born in 1974, Pharmacist |
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Keywords: | cardiomyocyte hypertrophy angiotensin II cyclosporine A c-fos Ca2+ |
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