| Effects of Triptolide on the Expression and Activity of NF-κB in Synovium of Collagen-induced Arthritis Rats |
| |
| 引用本文： | 涂胜豪,胡永红,曾克勤,张明敏,赖先阳,张玮琛.Effects of Triptolide on the Expression and Activity of NF-κB in Synovium of Collagen-induced Arthritis Rats[J].华中科技大学学报(医学英德文版),2005,25(5):543-545. |
| |
| 作者姓名： | 涂胜豪 胡永红 曾克勤 张明敏 赖先阳 张玮琛 |
| |
| 作者单位： | Department of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China |
| |
| 基金项目： | This project was supported by a grant from National Natural Sciences Foundation of China （No. 30070961）. |
| |
| 摘 要： | Summary： The expression and activity of NF-kB in the synovium of collagen-induced arthritis （CIA） rats was detected in order to investigate the possible therapeutic effects of triptolide on rheumatoid arthritis （RA）. The experimental Wistar rat model of CIA was set up by intradermal injection of emulsion of bovine collagen 11 and the successful rate of setting-up models was evaluated by arthritis index （AI）. Rats were grouped randomly into three groups： normal, model and treatment group. The expression of TNF-α and IL-6 in synovial fluid was detected by ELISA, and the expression and activity of NF kB in synovium by immunohistochemistry method and by electrophoretic mobility shift assay （EMSA） respectively. As compared with normal group, the expression of TNF a and IL-6 in synovia （P〈0. 05）, and the expression and activity of NF-kB （P〈0.05） in synovium were increased in model group. There was statistical difference in above-mentioned indexes between model group and treatment group. Triptolide may play a protective role in IRA via downregulating the expression and activity of NF-kB in synovium.
|
| 关 键 词： | 基因表达 NF-κB 滑膜 胶原质 关节炎 小鼠 动物实验 |
| 收稿时间： | 2004-06-21 |
Effects of triptolide on the expression and activity of NF-κB in synovium of collagen-induced arthritis rats |
| |
| Tu Shenghao,Hu Yonghong,Zeng Keqin,Zhang Mingmin,Lai Xianyang,Zhang Weichen.Effects of triptolide on the expression and activity of NF-κB in synovium of collagen-induced arthritis rats[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2005,25(5):543-545. |
| |
| Authors: | Tu Shenghao Hu Yonghong Zeng Keqin Zhang Mingmin Lai Xianyang Zhang Weichen |
| |
| Institution: | Department of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. |
| |
| Abstract: | The expression and activity of NF-kappaB in the synovium of collagen-induced arthritis (CIA) rats was detected in order to investigate the possible therapeutic effects of triptolide on rheumatoid arthritis (RA). The experimental Wistar rat model of CIA was set up by intradermal injection of emulsion of bovine collagen II and the successful rate of setting-up models was evaluated by arthritis index (AI). Rats were grouped randomly into three groups: normal, model and treatment group. The expression of TNF-alpha and IL-6 in synovial fluid was detected by ELISA, and the expression and activity of NF-kappaB in synovium by immunohistochemistry method and by electrophoretic mobility shift assay (EMSA) respectively. As compared with normal group, the expression of TNF-alpha and IL-6 in synovia (P < 0.05), and the expression and activity of NF-kappaB (P < 0.05) in synovium were increased in model group. There was statistical difference in above-mentioned indexes between model group and treatment group. Triptolide may play a protective role in RA via downregulating the expression and activity of NF-kappaB in synovium. |
| |
| Keywords: | |
| 本文献已被 CNKI 维普 SpringerLink 等数据库收录！ |
