Expression and biological function of N-myc down-regulated gene 1 in human cervical cancer |
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Authors: | Jing Wang Jing Cai Zhimin Li Sha Hu Lili Yu Lan Xiao Zehua Wang |
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Institution: | 1. Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China 2. Department of Obstetrics and Gynecology, the Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China |
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Abstract: | The expression of N-myc down-regulated gene 1 (NDRG1) has previously been reported to be involved in the proliferation, differentiation,
invasion and metastasis of cancer cells, but its role in cervical cancer is still unclear. This study aimed to investigate
the expression of NDRG1gene in human cervical cancer and its effect on aggressive tumor behaviors. The NDRG1 expression in
cervical tissues and cells was detected by RT-PCR. Specific expression plasmid pEGFP-N1-NDRG1-GFP was used to enhance the
expression of NDRG1 in human cervical cancer cell lines. The mRNA and protein level of NDRG1 was assessed by RT-PCR and Western
blotting, respectively. Its effects on cell proliferation, migration, invasion, cell cycle and apoptosis were detected by
MTT, transwell migration assay and flow cytometry (FCM), respectively. The results showed that the expression of NDRG1 in
cervical cancer tissues and cells was significantly lower than in normal cervical tissues (P<0.001). After transfection with pEGFP-N1-NDRG1-GFP, the mRNA and protein expression of NDRG1 was up-regulated in Siha cells,
which suppressed cell proliferation (P<0.001), induced cell cycle arrest (P<0.05), reduced invasion and migration of Siha cells (P<0.05), but caused no cell apoptosis. Moreover, vascular endothelial growth factor (VEGF), a tumor-induced angiogenesis factor,
was markedly reduced and E-cadherin, a cell adhesion molecule, was increased in the cells transfected with pEGFP-N1-NDRG1-GFP.
It was concluded that up-regulated NDRG1 may play a role in the suppression of malignant cell growth, invasion and metastasis
of human cervical cancer. |
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Keywords: | N-myc down-regulated gene 1 cervical cancer transfection cell proliferation invasion |
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