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Establishment of Germ-line Competent C57BL/6J Embryonic Stem Cell Lines
作者姓名:Gui-jun  YAN  Zheng  GU  Jian  WANG  Jia-ke  TSO
作者单位:[1]Fudan University Medical School, Shanghai 200032, China [2]Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China
摘    要:Objective To establish C57BL/6J embryonic stem (ES) cell lines with potential germ- line contribution Methods ES cells were isolated from blastocyst inner cell mass of C5 7BL/6J mice, and cultured for 15 passages, and then injected into blastococels of ICR mice blastocysts to establish chimeric mice. Results Three ES cell lines (mC57ES1,mC57ES3, mC57ES7) derived from the inner cell mass of C57BL/6J mice blastocysts were established. They were characteristic of undifferentiated state, including normal XY karyotype, expression of a specific cell surface marker “stage-specific embryonic antigen-I” and alkaline phosphatase in continuous passage. When injected into immunodeficient mice, mC57ES1 cells consistently differentiated into derivatives of all three embryonic germ layers. When mC57ES1 cells were transferred into ICR mice blastocysts, 4 chimeric mice have been obtained. One male of them revealed successful germ-line transmission. Conclussion We have obtained C57BL/6J ES cell lines with a potential germ-line contribution, which can be used to generate transgenic and gene knock-out mice.

关 键 词:细菌感染  C57BL/6J  胚胎干细胞  小鼠  动物实验  临床研究

Establishment of Germ-line Competent C57BL/6J Embryonic Stem Cell Lines
Gui-jun YAN Zheng GU Jian WANG Jia-ke TSO.Establishment of Germ-line Competent C57BL/6J Embryonic Stem Cell Lines[J].Journal of Reproduction and Contraception,2004,15(4):201-208.
Authors:Gui-jun YAN  ZHENG GU  Jian WANG  Jia-ke TSO
Abstract:Objective To establish C57BL/6J embryonic stem (ES) cell lines with potential germline contribution Methods ES cells were isolated from blastocyst inner cell mass of C57BL/6J mice, and cultured for 15 passages, and then injected into blastococels of lCR mice blastocysts to establish chimeric mice.Results Three ES cell lines (mC57ESl,mC57ES3, mC57ES7) derived from the inner cell mass of C57BL/6J mice blastocysts were established. They were characteristic of undifferentiated state, including normal XY karyotype, expression of a specific cell surface marker "stage-specific embryonic antigen-1" and alkaline phosphatase in continuous passage. When injected into immunodeficient mice, mC5 7ES1 cells consis tently differentiated into derivatives of all three embryonic germ layers. When mC57ES1cells were transferred into ICR mice blastocysts, 4 chimeric mice have been obtained.One male of them revealed successful germ-line transmission. Conclussion We have obtained C57BL/6J ES cell lines with a potential germ-line contribution, which can be used to generate transgenic and gene knock-out mice.
Keywords:C57BL/6J mice  embryonic stem (ES) cell  ICR mice blastocysts  germ-line  competent
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