Correlation of IL-1, IL-6, IL-10 Concentrations to Ovarian Hyperstimulation Syndrome and Effect of Intravenous Immunoglobulin on Ovarian Hyperstimulated Rats

Objective To investigate the correlation of interleukin(IL)-1,IL-6 and IL-10 concen-trations to ovarian hyperstimulation syndrome(OHSS) and whether intravenousimmunoglobulin(IVIG) has the effects on ovarian hyperstimulated rats. Methods Immature female Wistar rats were divided into control group,OHSS group (n=13)and IVIG group(n=13).For the latter two groups,pregnancy mare serum gonadotropin(PMSG)and human chorionic gonadotropin(hCG)were given to induce OHSS,and rats in IVIG group were treated with immunoglobulin.Forty—eight hours after administration of hCG,capillary permeability was evaluated from the Evans blue dye(EB) concentration in the ovaries and the EB concentration in peritoneal irrigated fluid at 30 min after the intravenous injection of EB.Rats’blood samples and ovaries were obtained to be measured for IL-1,IL-6 and IL-10 by ELISA.Results In OHSS group,total weights of bilateral ovaries and the ovarian EB concen—tration were significantly higher than those in others(P<0.05).Both serum and ovarian concentrations of IL-1 were significantly higher in OHSS and IVIG groups than those in control group (P<0.05).The ovarian concentrations of IL-6 and IL-10 in IVIG group were significantly lower than those in control group(P<0.05).Furthermore,the ovarian IL-10 concentration in IVIG group was significantly lower than that in OHSS group(P<0.05). Conclusion Inflammation involved IL-1 in OHSS rats plays an important role.Vascular permeability was mostly increased in ovaries of hyperstimulated rats.It appears that ovaries of OHSS rats may be the primary places of inflammation.IVIG treatment resulted in statistically significant reductions in ovaries’weights and ovarian vascular permeability of OHSS rats,with a decreased level of ovarian IL-10.It implys that IVIG have a beneficial effect in reducing the severity of OHSS in the experimental model maybe by restrainning IL-10.