和颜坤泰胶囊对卵巢早衰大鼠性激素水平和VEGF、bFGF mRNA表达的影响

目的 探讨中成药坤泰胶囊治疗不同阶段卵巢早衰的效果.方法 采用雷公藤多苷片75mg/kg制作卵巢早衰大鼠模型;将60只SD雌性大鼠分别为空白组,模型组,造模前坤泰胶嚢组(造模前组),造模中坤泰胶囊组(造模中组),造模后坤泰胶囊组(造模后组),西药组(结合雌激素治疗).观察大鼠行为学的改变,采用酶联免疫吸附试验(ELISA)法检测卵泡刺激素(FSH)、黄体生成素(LH)、血清雌二醇(E2)及抗缪勒管激素(AMH)的水平.RT-PCR法检测卵巢组织中血管内皮生长因子(VEGF)、碱性成纤维细胞生长因子(bFGF) mRNA的表达.结果 空白组和各给药组血清FSH及LH水平明显低于模型组(P＜0.05),E2、AMH水平明显高于模型组(P＜0.05).空白组及各给药组卵巢组织中VEGF、bFGF mRNA的表达明显高于模型组(P＜0.05),其中造模前组VEGF、bFGF mRNA水平高于造模中组、造模后组(P＜0.05),造模前组VEGF mRNA的表达与西药组比较差异无统计学意义(P＞0.05).结论 坤泰胶囊对卵巢性激素水平具有调节作用,能提高血清E2及AMH的水平,降低血清FSH、LH的水平,提高卵巢对性激素水平的敏感性;修复受损的卵巢组织,促进卵泡的生长发育,从而抑制卵泡过早耗竭.

Effect of Yan Kuntai capsule on rat sex hormone level and VEGF bFGF mRNA expression in different stages and premature ovarian failure

Objective To explore the Chinese medicine treatment of QUNTAI capsule in different stages of premature ovarian failure effect.Methods Sixty SD female rats were divided into 6 groups:normal group,model group,prevention group,intervention group,the treatment group,and Western medicine group.To observe the changes of behavior in rats,enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of follicle stimulating hormone FSH,LH,serum estradiol E2,and anti Mueller hormone AMH.RT-PCR was used to detect ovarian tissue vascular endothelial growth factor (VEGF) and basic effect of fibroblast growth factor (bFGF) mRNA expression.Results The levels of FSH and LH in the normal group and the administration group were significantly lower compare to model group (P＜0.05),and the contents of AMH and E2 were significantly higher compare to model group (P＜0.05).The FSH and LH content in the model group was significantly higher than that of the normal group and each treatment group,E2,AMH content was significantly lower than that of the normal group and each treatment group (P＜0.05);the expression of VEGF andbFGF mRNA in each treatment group and nornial group was significantly higher than that of model group (P＜0.05),Prevention group was higher than intervention group and treat ment group (P＜0.05),there was no Gignificont difference between prevention group and Western medicine group(P＞0.05).Conclusion QUNTAI capsule can increase serum E2 and AMH levels,lower serum FSH,LH levels,improve ovarian sensitivity to sex hormone levels,repair damaged ovarian tissue,promote follicular growth and development,Thereby inhibiting premature follicle depletion.