黄芩苷对UUO大鼠肾间质纤维化的影响及可能机制

目的 探讨黄芩苷对单侧输尿管梗阻(UUO)模型肾间质纤维化(RIF)的影响及可能机制.方法 40只雄性SD大鼠分为假手术组、模型组、黄芩苷组、贝那普利组及黄芩苷+贝那普利组,每组各8只.黄芩苷组给予黄芩苷溶液灌胃40 mg·kg-1·d-1,贝那普利组给予贝那普利混悬液灌胃10 mg·kg-1·d-1,黄芩苷+贝那普利组给予黄芩苷溶液40 mg·kg-1·d-1和贝那普利混悬液10 mg·kg-1·d-1灌胃,假手术组与模型组大鼠给予等量生理盐水灌胃.在造模第14、21和28天处死动物,取肾脏标本,免疫组化法检测肾组织转化生长因子-β1 (TGF-β1)和α-平滑肌肌动蛋白(α-SMA)的表达.结果 与假手术组相比,模型组大鼠在造模第14、21和28天,肾组织内TGF-β1、α-SMA的表达均明显升高(P＜0.05).与模型组相比,黄芩苷组、贝那普利组及黄芩苷+贝那普利组大鼠在造模第14、21和28天,肾组织内TGF-β1、α-SMA的表达均不同程度降低(P＜0.05).而黄芩苷组、贝那普利组和黄芩苷+贝那普利组3组之间各时间点肾组织内TGF-β1、α-SMA的表达两两比较差异均无统计学意义(P＞0.05).结论 黄芩苷可能通过下调TGF-β1、减少α-SMA表达,从而延缓UUO大鼠RIF的进展.

Effects and possible mechanism of baicalin on renal interstitial fibrosis in unilateral urethral obstruction rat

Objective To explore the effects and possible mechanism of baicalin on renal interstitial fibrosis in unilateral urethral obstruction (UUO) rat.Methods Forty male SD rats were randomly divided into the sham operation group,model group,baicalin group,benazepril group and baicalin+ benazepril group,8 rats in each group.The baicalin group received the gavage of baicalin 40 mg · kg-1 · d 1,the benazepril group received the gavage of benazepril suspension 10 mg · kg-1 · d-1,the rats baicalin+ benazepril group received the gavage of baicalin 40 mg · kg-1 · d 1 and benazepril suspension 10 mg · kg-1 · d-1,the model group and sham operation group were given the same volume of normal saline.Rats were sacrificed in each group on 14,21,28 d after model construction and the kidney sample was obtained.Immunohistochemistry was used to detect the expression of TGF-β1 and α-SMA.Results Compared with the sham operation group,the TGF-β1 and α-SMA expression levels of renal tissue in the model group were significantly increased(P＜0.05),compared with the model group,the TGF-β1 and α-SMA expression levels on 14,21,28 d of model construction in the baicalin group,baicalin+ benazepril group and benazepril group were decreased to different extents (P＜0.05).But the TGF-β1 and α-SMA levels at each time point had no statistical difference among the baicalin group,benazepril group and baicalin+benazepril group(P＞0.05).Conclusion Baicalin may delay the progress of renal interstitial fibrosis in UUO rat by down-regulating the TGF-β1 expression and reducing α-SMA expression.