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VEGF基因真核表达载体的构建及其在骨髓间充质干细胞中的表达
引用本文:张从纪,杨彦春,单佑安.VEGF基因真核表达载体的构建及其在骨髓间充质干细胞中的表达[J].重庆医学,2010,39(1).
作者姓名:张从纪  杨彦春  单佑安
作者单位:第三军医大学西南医院口腔科,重庆,400038
基金项目:国家自然科学基金资助项目(30670636);;重庆市自然科学基金资助项目(2006BB5095)
摘    要:目的探讨经腺病毒表达载体转染血管内皮细胞生长因子(vascular endothelial growth factor,VEGF)165基因的骨髓间充质干细胞(mesenchymal stemcells,MSCs)体内外基因表达的特点。方法构建VEGF基因腺病毒表达载体,将VEGF165基因转染到MSCs中。用RT-PCR法检测转基因MSCs中基因表达情况。结果转基因MSCs及其分化的子代细胞均有VEGF165的表达,并持续2周左右时间。结论经腺病毒表达载体转染VEGF165基因的MSCs在体内外均有良好的基因表达。

关 键 词:血管内皮细胞生长因子  骨髓间充质干细胞  基因转移  

Construction of vascular endothelial growth factor gene eukaryotic expression vector and its expression in mesenchymal stem cells
ZHANG Cong-ji,YANG Yan-chun,SHAN You-an.Construction of vascular endothelial growth factor gene eukaryotic expression vector and its expression in mesenchymal stem cells[J].Chongqing Medical Journal,2010,39(1).
Authors:ZHANG Cong-ji  YANG Yan-chun  SHAN You-an
Institution:Department of Stomatology;Southwest Hospital;Third Military Medical University;Chongqing 400038;China
Abstract:Objective To detect the gene expression of rat mesenchymal stem cells(MSCs) transfected with vascular endothelial growth factor(VEGF)165 mediated by pAdTrack.Methods Construction of VEGF gene eukaryotic expression vector,and VEGF gene was transferred to MSCs.Gene expression of transfected cells was detected by RT-PCR.Results VEGF transfected MSCs could continuously express gene products during the first 2 weeks.Conclusion MSCs transfected with VEGF gene by pAdTrack can express gene products efficiently in v...
Keywords:vascular endothelial growth factor  mesenchymal stem cells  gene transfection  
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