| survivin特异性RNAi表达载体的构建及其干扰效果鉴定 |
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| 引用本文： | 汪欣,杨军,许波,熊宇,张从纪.survivin特异性RNAi表达载体的构建及其干扰效果鉴定[J].重庆医学,2009,38(14). |
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| 作者姓名： | 汪欣 杨军 许波 熊宇 张从纪 |
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| 作者单位： | 第三军医大学西南医院口腔科,重庆,400038 |
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| 摘 要： | 目的 构建survivin基因的RNAi真核表达载体并观察其对转染腺样囊性癌细胞株ACC-2后survivin表达变化以及对细胞凋亡的影响.方法 设计、合成siRNA(small interfering RNA),构建表达载体pGenesil-shRNA-survivin,将重组质粒导入人腺样囊性癌ACC-2细胞株.采用逆转录-聚合酶链反应(RT-PCR)法检测转染后的ACC-2细胞中survivin mRNA表达的变化,用TUNEL检测细胞凋亡的变化.结果 经测序模版序列和设计序列完全正确,在pGenesil-shRNA-survivin转载ACC-2细胞株中,survivin mRNA表达明显降低,转染的ACC-2细胞凋亡显著增加.结论 成功构建了survivin基因的RNAi真核表达载体,且其对腺样囊性癌细胞有一定的干扰效果.
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| 关 键 词： | 质粒 |
Construction and effect identification of RNAi eukaryotic expression vectors of survivin* |
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| Abstract: | Objective To investigate apoptosis effect of siRNA on survivin in Adenoid cystic carcinoma cells lines.Methods siRNA was designed and synthesized.Eukaryotic expression vector pGenesil-shRNA-survivin was constructed.Recombinant Plasmid was transfected into ACC-2 cells.The expression of survivin mRNA was detected by RT-PCR and cell apoptosis was detected by TUNEL.Results The recombinant plasmid was successfully constructed.The expression of survivin mRNA was obviously reduced.ACC-2 cells apoptosis was promote... |
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| Keywords: | survivin RNAi |
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