抑制survivin基因表达对肺腺癌A549细胞增殖和凋亡的影响

目的研究survivin基因与肺腺癌的关系,探讨survivin对肺腺癌A549细胞增殖和凋亡的影响。方法合成抑制survivin基因的siRNA,通过脂质体转染到肺腺癌A549细胞中,荧光倒置显微镜下观察细胞形态学变化,流式细胞仪检测各组细胞增殖和凋亡指数,Western blot检测survivin编码表达的蛋白,RT-PCR检测survivin mRNA的表达。结果抑制survivin在肺腺癌A549细胞中的表达后,能够抑制A549细胞增殖,诱导细胞的凋亡,survivin基因编码表达的蛋白明显减少。结论应用RNAi抑制survivin基因,可以抑制A549细胞增殖,促进细胞凋亡。survivin能作为治疗肺腺癌的一个潜在的基因靶点。

Effect of inhibition of survivin on the cell proliferation and apoptosis of adenocarcinomaof lung cell lines A549 cell

Objective To explore the relation between survivin and lung adenocarcinoma,and approach to the effect of survivin on lung adenocarcinoma cell lines A549 proliferation and apoptosis.Methods survivin siRNA was synthetized and transfected into A549 cell by liposome.Cell morphological transformation was observed with inverted fluorescent microscope,cell proliferation and apoptotic index were detected by flow cytometry,the proteinum expressed by survivin was detected by western blot and survivin mRNA expression was determined by RT-PCR.Results For the A549 cell transfected with siRNA cell proliferation was inhibited significantly,cell apoptosis appeared obviously and proteinum expressed by survivin significantly decreased after treatment with siRNA.Conclusion RNAi is able to inhibit the proliferation of lung adenocarcinoma cell lines A549 and induce the cell apoptosis.survivin might provide a potential target for lung adenocarcinoma gene therapy.