壳多糖-胶原-糖胺聚糖凝胶人工皮肤的制备

目的 研制一种新型的凝胶类人工皮肤。方法 制备壳多糖－胶原－糖胺聚糖（GAGs)－成纤维细胞真皮替代物（DE），随后在“成熟”的DE表面接种KC，先浸没培养，再气液界面培养，构建完整的人工皮肤。对人工皮肤行组织学分析。结果 人工真皮浸没培养将发生一定程度的收缩，接种角质形成细胞将促进其收缩。液气界面培养一定时间后的人工皮肤有结构致密的真皮和分化良好的表皮。结论 我们制作的壳多糖－胶原－GAGs凝胶人工皮肤是有满意真皮表结构的新型人工皮肤。

Constitution of the chitosan-collagen-GAGs collagen gel artificial skin

Objective To constitute a new collagen gel artificial skin.Methods Human foreskin FBs were incorporated into the chitosan collagen GAGs matrix to constitute dermal equivalent(DE).KCs separated from children foreskin were then seeded on the matured DE to reconstruct artificial skin, which was immersed at the early stage, followed by lifting to an air liquid interface.The structure of composite chitosan DE and SE were analysed by histology.Results The immersing cultured artificial dermis contracted partially, and it contracted faster after implanting keratinocytes.The artificial skin has density dermis and good differentiated epidermis after cultured in air liquid interface.Conclusion The composite chitosan artificial skin made by us was a satisfactory skin substitute with dermis and epidermis.