| COX-2信号通路参与高糖高胰岛素诱导的心肌细胞肥大研究 |
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| 引用本文： | 李劲松.COX-2信号通路参与高糖高胰岛素诱导的心肌细胞肥大研究[J].重庆医学,2011,40(25):2537-2538,2540. |
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| 作者姓名： | 李劲松 |
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| 作者单位： | 李劲松 (重庆市双桥区人民医院,400900) ； |
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| 摘 要： | 目的探讨环氧化酶-2(COX-2)在高糖高胰岛素诱导的心肌肥大中的作用。方法用高糖高胰岛素刺激体外培养的乳鼠心肌细胞,以细胞表面积、蛋白含量和心房利钠因子(ANF)mRNA表达为心肌细胞肥大的反映指标,观察COX-2特异性抑制剂———赛来昔布对高糖高胰岛素致肥大作用的影响。利用real-time PCR检测细胞中mRNA的表达。结果高糖高胰岛素诱导细胞表面积、总蛋白含量以及ANF、COX-2 mRNA的表达增加(P<0.05);赛来昔布可以抑制高糖高胰岛素诱导的心肌细胞肥大(P<0.05),同时抑制COX-2的表达(P<0.05)。结论赛来昔布可以通过抑制COX-2的表达,从而对抗高糖高胰岛素诱导的心肌肥大。
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| 关 键 词： | 糖尿病 环氧化酶-2 心肌细胞肥大 高糖高胰岛素 |
Study on influence of COX-2 in cardiomyocyte hypertrophy induced by high glucose and insulin |
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| Li Jinsong.Study on influence of COX-2 in cardiomyocyte hypertrophy induced by high glucose and insulin[J].Chongqing Medical Journal,2011,40(25):2537-2538,2540. |
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| Authors: | Li Jinsong |
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| Institution: | Li Jinsong(People′s Hospital of Shuangqiao District,Chongqing 400900,China) |
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| Abstract: | Objective To investigate the effects of clooxygenase 2(COX-2) in cardiomyocyte hypertrophy induced by high glucose and insulin.Methods Use high glucose and insulin to activate the cultured neonatal rat cardiomyocytes,and take the cell surface area,protein content,and atrial natriuretic area(ANF) mRNA expression as the indexes of cardiomyocyte hypertrophy,and then observe the effect of COX-2 on cardiomyocyte hypertrophy caused by high glucose and insulin.real-time PCR was used to assay the expression of COX-... |
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| Keywords: | Diabetes mellitus clooxygenase 2 cardiomyocyte hypertrophy high glucose and insulin |
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