PLGA神经诱导管的制作及其神经再生诱导作用

目的观测制备的聚乳酸-聚羟基乙酸共聚物Poly(DL-lactide-co-glycolide)PLGA]管的神经再生诱导作用.方法采用大鼠坐骨神经缺损模型观察以两种构成比不同(85:15)或(50:50)]的聚乳酸-聚羟基乙酸共聚物制备的PLGA管的神经再生诱导作用.结果(1)体内神经桥接情况下,硅胶管促发有明显的纤维组织增生,并形成包囊包裹于管壁,而两种PLGA管组未见类似现象;(2)与硅胶管组相似,PLGA(85:15)管能够顺利完成坐骨神经缺损桥接作用,再生神经电生理及组织学评价显示两组无明显差异,而PLGA(50:50)管体内神经桥接4周时即出现破裂,不能为坐骨神经桥接提供良好的支持作用.结论与硅胶管及PLGA(50:50)管相比,PLGA(85:15)管能够提供良好的神经再生诱导作用,而且由于其并物反应小、生物相容性佳,生物降解率适当,是一种良好的周围神经组织工程材料.

Nerve guidance effects of poly(DL-lactide-co-glycolide) conduits

Objective To investigate the effects of poly(DL-lactide-co-glycolide) ] (PLGA) conduits on nerve guidance.Methods The effects of two different copolymer ratio of PLGA (85:15) and (50:50)] conduits on bridging sciatic nerve defects in rats were evaluated.Results (1)Silicone induced significant proliferation of fibre tissue, characterized by encapsulation,when bridging sciatic nerve defects in rats, but the similar response can not be observed in PLGA in the same situation. (2)Similar to silicone tube group,the PLGA(85:15) conduits maintained a stable support for axon regeneration for up to 12 weeks in sciatic nerve defects bridging model in rats . However, PLGA(50:50) conduit collapsed at 4 week in the same animal model.Conclusion The PLGA(85:15) is good material for peripheral nerve tissue engineering with good biocompatibility and biodegradability.