亚甲蓝血浆病毒灭活对凝血因子的影响

目的探讨亚甲蓝(MB)光化学法血浆病毒灭活对主要凝血因子活性的影响,以及血浆病毒灭活后制备冷沉淀的可行性。方法将16袋400mL全血成分分得的新鲜血浆均分为两份,分别归为实验组和对照组;实验组加入MB进行病毒灭活,对照组不加MB但仍然进行病毒灭活光照过滤,检测各阶段标本凝血因子Ⅷ(FⅧ):C及纤维蛋白原(Fib)。结果实验组与对照组之间FⅧ:C及Fib差异有统计学意义(P<0.05),经过病毒灭活后实验组血浆中FⅧ及Fib损失较大且含量未达制备冷沉淀的理论要求。结论血浆病毒灭活对血浆中FⅧ及Fib均有较为显著的影响,且其损耗主要来自MB光化学反应,血浆病毒灭活后不宜用于制备冷沉淀。

The influence of methylene blue virus inactivation on coagulation factor in plasma

Objective To investigate the effect of methylene blue photochemical plasma viral inactivation on the activity of main coagulation factors,and the feasibility that the preparation of cryoprecipitate with viral inactivation plasma.Methods 16 prepared plasma were equally splited into two aliquots,namely experimental group and control group respectively.The plasma of experimental group was inactivated with methylene blue light treatment,and the plasma of control group was treated with light without methylene blue.FⅧ and Fib concentration of specimens were analyzed.Results The difference of FⅧ and Fib was significant between the two groups(P<0.05).The loss of FⅧ and Fib in the experimental group plasma was too large to prepare the cryoprecipitate.Conclusion The effect of plasma viral inactivation on FⅧ and Fib was significant,and was mainly by the photochemical reaction of methylene blue.Virus inactivated plasma is not appropriate for the preparation of cryoprecipitate.