前胡丙素对自发性高血压大鼠血压及心肌受磷蛋白表达的影响

目的探讨前胡丙素对自发性高血压大鼠(SHR)血压的影响及可能机制。方法选择SHR 20只,随机分为药物组(n=10),对照组(n=10),另选SD大鼠10只为SD组。药物组给予前胡丙素20mg/kg-1.d-1灌胃,其余对照组和SD组同等容积聚乙二醇400灌胃,干预8周,运用透射电镜观察心肌细胞超微结构及线粒体损伤情况,并用半定量反转录PCR(RT-PCR)法测定受磷蛋白(PLB)的基因表达水平。结果治疗前药物组大鼠的收缩压(184.3±19.7)mm Hg]与对照组(183.3±17.2)mmHg]接近,与SD组相比明显升高,差异有统计学意义,治疗后药物组大鼠收缩压(149.3±12.6)mm Hg]比对照组大鼠心肌(201.0±11.0)mm Hg]低(P<0.05),对照组PLB表达水平与SD组相比有明显的降低,治疗后药物组大鼠心肌PLB的表达较对照组上调(P<0.05)。结论前胡丙素有一定的降压作用,可能是通过上调SHR心肌肌质网PLB的基因表达。

Effects of praeruptorin C on blood pressure and expression of myocardial phospholamban in spontaneously hypertensive rats*

Objective To explore the impact and the possible mechanism of prearuptorin C(Pra-C) on blood pressure in spontaneously hypertensive rats(SHR).Methods 20 spontaneously hypertensive rats were randomly divided into the Pra-C group treated with Pra-C 20 mg/(kg-1·d-1) by gastrogavage(n=10) and untreated control group(n=10).The untreated control group and 10 age matched Sprague Dawley rats as SD group received gastrogavage with same volume of polyethylene glycol.Each rat in various groups was intervened for 8 weeks.The lesions of ultrastructure and mitochondria were observed by transmission electron microscope.The gene expression levels of phospholamban(PLB) were determined by the method of semiquantitative RT-PCR.Results Before treatment,the systolic pressure of SHR in the Pra-C group was(184.3±19.7)mm Hg,which was closed to(183.3±17.2)mm Hg in the control group,but significantly higher than that in the SD group.After treatment,the systolic pressure of SHR in the Pra-C group was(149.3±12.6)mm Hg,which was obviously lower than(201.0±11.0)mm Hg in the control group(P<0.05).Compared with the SD group,the expression of PLB in the control group was significantly reduced,but which after treatment in the Pra-C group was upregulated compared with the control group(P<0.05).Conclusion Pra-C has a certain hypotensive effect by upregulating the gene expression of myocardial sarcoplasmic reticulum PLB.