| 2种不同类型的核酸探针对压电基因传感器阵列检测特异性的影响 |
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| 引用本文： | 吴蓉,府伟灵,陈鸣.2种不同类型的核酸探针对压电基因传感器阵列检测特异性的影响[J].重庆医学,2004,33(8):1135-1137. |
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| 作者姓名： | 吴蓉 府伟灵 陈鸣 |
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| 作者单位： | 第三军医大学西南医院检验科,重庆,400038;第三军医大学西南医院检验科,重庆,400038;第三军医大学西南医院检验科,重庆,400038 |
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| 基金项目： | 国家高技术研究发展计划(863计划)
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国家科技攻关项目
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国家自然科学基金
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军队科技攻关项目
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军队科技攻关项目
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重庆市应用基础研究基金
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重庆市科委风险投资基金 |
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| 摘 要： | 目的探讨用PNA作为探针与普通的DNA探针相比对压电传感器基因杂交的优越性.方法用生物素-亲和素法分别将PNA、DNA两种探针固定在基因传感器的金膜表面,分别与完全匹配、1个碱基错配、2个碱基错配的互补靶序列进行杂交,观察2种不同的探针与靶序列反应所引起的频率变化及所用的时间.结果相对于DNA探针,PNA探针识别碱基错配的能力明显高,且杂交时间更短.结论 PNA与靶序列DNA结合有高度的特异性,选用PNA作探针,可提高压电基因传感器检测的特异性.
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| 关 键 词： | 肽核酸 核酸杂交 传感器 压电晶体 |
| 文章编号： | 1671-8348(2004)08-1135-03 |
Influence of two different types of nucleic acid probes on detection for specificity of piezoelectric gene sensor array |
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| WU Rong,FU Wei ling,CHEN Ming.Influence of two different types of nucleic acid probes on detection for specificity of piezoelectric gene sensor array[J].Chongqing Medical Journal,2004,33(8):1135-1137. |
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| Authors: | WU Rong FU Wei ling CHEN Ming |
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| Abstract: | Objective To investigate the superiority of the gene hybridize of the piezoelectric sensor by comparing the probe of PNA with the ordinary probe of DNA.Methods The probe of PNA and DNA were immobilized on the gold surface with bioth avidin method,and hybridized with target sequence of the complete,one mismatch and two mismatch sequence,respectively.Observe the change of the frequency and the cost time of the reaction of the two different probes.Results The recognizing mismatch base capability of PNA probe was high and the cost time of hybridization was shorter.Conclusion PNA combining with the DNA of target sequence had a highly specificity.Choosing PNA as the probe may improve the determined specificity of piezoelectric gene sensor. |
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| Keywords: | peptide nucleic acid(PNA) nucleotide hybridization sensor piezoelectric crystal |
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