电场对内皮祖细胞迁移行为及形态的影响

目的：探讨外加电场对体外培养的内皮祖细胞（EPCs）迁移行为及形态的影响。方法将体外培养的3～4代EPCs分别用场强为0 mV／mm（Ⅰ组）、100 mV／mm（Ⅱ组）、200 mV／mm（Ⅲ组）和300 mV／mm（Ⅳ组）的直流电场加以持续刺激3 h ，活细胞工作站实时记录细胞迁移轨迹及形态变化，分析外加电场对EPCs迁移行为及形态的影响。结果Ⅳ、Ⅲ、Ⅱ组细胞向阳极定向迁移，Ⅰ组细胞显示随机运动。电刺激3 h时，细胞轨迹迁移速率（Vt）、位移速率（Vd）、电场方向迁移速率（Vx）分别为：Ⅳ组（98．86±6．00）、（63．78±2．81）、（63．15±2．88）μm／h]、Ⅲ组（88．06±8．83）、（35．90±1．22）、（34．20±1．57）μm／h]和Ⅱ组（42．28±2．25）、（13．29±0．37）、（12．39±0．51）μm／h]，均显著高于Ⅰ组的（37．39±2．42）、（6．99±0．31）、（4．62±0．40）μm／h]，均 P＜0．01，且Ⅲ组细胞Vt、Vd、Vx均显著高于Ⅱ组、Ⅰ组（均 P＜0．01）。EPCs在电场刺激下发生明显的形态变化，细胞外观变得狭长，逐渐与电场方向平行排列。结论外加直流电场可诱导EPCs向阳极迁移并加快其迁移速率，且对EPCs的形态有明显影响。

Influence of electric fields on migration behavior and morphology of endothelial progenitor cells

Objective To investigate the influence of external electric fields on migration behavior and morphology of endo‐thelial progenitor cells (EPCs) cultured in vitro .Methods The in vitro cultured 3-4 generation EPCs were continuously stimula‐ted by direct‐current electric field with the field intensity of 0 mV/mm(group Ⅰ ) ,100 mV/mm(group Ⅱ) ,200 mV/mm(group Ⅲ) and 300 mV/mm (group Ⅳ )for 3 h .The live cell station was used to real time record the cell migration track and morphology change of EPCs .The influence of external electric field on the EPCs migration behavior and morphology was analyzed .Results Un‐der the stimulation of the direct‐current electric field with the intensity of group Ⅳ ,group Ⅲ and group Ⅱ ,the cells were directly migrated to anode ,while the cells under group Ⅰ displayed the random motion .The track migration velocity(Vt)、displacemnt ve‐locity(Vd) and electric field direction migration rate(Vx) were(98 .86 ± 6 .00) ,(63 .78 ± 2 .81) ,(63 .15 ± 2 .88)μm/h for the groupⅣ ,(88 .06 ± 8 .83) ,(35 .90 ± 1 .22) ,(34 .20 ± 1 .57)μm/h for the groupⅢ ,(42 .28 ± 2 .25) ,(13 .29 ± 0 .37) ,(12 .39 ± 0 .51)μm/h for the groupⅡ ,which were significantly higher than(37 .39 ± 2 .42) ,(6 .99 ± 0 .31) ,(4 .62 ± 0 .40)μm/h for the groupⅠ (P<0 .01) ,moreover Vt ,Vd and Vx in the group Ⅲ were significantly higher than those in the group Ⅱ andⅠ (P<0 .01) .EPCs had obvious morphological changes under the electric field action ,such as elongation and the cellular long axis parallel to the electric field direction .Conclusion External direct current electric fields may induce the directed migration of EPCs towards the anode ,ac‐celerates the migration rate ,moreover has obvious influence on EPCs morphology .