CO2激光用于微流控免疫分析芯片加工的初步研究

目的建立微流控芯片的CO2激光直接微加工方法。方法构建激光加工装置，设置激光微加工程序，并对激光加工过程中一些影响因素进行分析，最后将加工后的芯片用于免疫分析。结果CO2激光可用于微流控芯片的加工，微流控芯片通道的深度主要受激光加工强度和激光加工次数的影响。对微通道表面的修饰可以改善通道的光滑度，采用该方法加工后的芯片可以用于免疫分析。结论CO2激光微加工法是一种操作方便、所需设备简单、加工周期短的微流控芯片加工方法。

Study on micromachining of immunoassay microfluidic chip by CO2 laser

Objective To establish direct-write laser method for micromachining immunoassay microfluidic chip.Methods To construct direct-write laser micromachining system,set micromachining program,analyse the factor of micromachining,and use the microfluidic chip to detect alpha-fetoprotein.Results CO_2 laser could be used in microfluidic chip micromachining,the depth of microchannel was affected by laser intensity and the times of micromachining,modification of microchannel could be improve it's smoothness.The microfluidic chip microfabricated by this method could be used in immunoassay.Conclusion Direct-write laser method is simple,convenient and speedy for microfluidic chip micromachining.