苦参碱对K562细胞增殖及凋亡相关分子表达的影响

目的 研究苦参碱对人白血病细胞K562增殖、凋亡的影响及其机制。方法 苦参碱（0.2mg/ml）作用于K562细胞，每日计数白细胞、计算抑制率；流式细胞分析仪检测苦参碱对K562细胞周期的改变及凋亡的影响；电子显微镜观察凋亡细胞形态；Western blot检测BCL－6及增列细胞核抗原（PCNA）表达情况。结果 苦参碱对K562细胞有明显的抑制作用，用药72h的细胞增殖抑制率达69％；用药5d后，G1期细胞明显增多，高达58.5%，68％的细胞发生凋亡。电子显微镜可观察到苦参碱诱导的早、中、晚期K562凋亡细胞；BCL－6表达增强，PCNA表达下降。结论 苦参碱能显著抑制K562细胞增殖、促进其凋亡，其机制可能与细胞增殖周期受阻及PCNA表达下降、BCL－6表达增加有关。

Effects of matrine on the relative molecules expression of proliferation and apoptosis in K562 cells

OBJECTIVE: To study the effects of matrine on proliferation and apoptosis of K562 cells. METHODS: Cell culture and cell count, cell cycle phases and number of apoptotic cells were analyzed by flow cytometry. Morphology of apoptotic cells was studied by electron microscopy, BCL-6 and proliferating Cell nuclear antigen (PCNA) were checked by Western blot. RESULTS: Matrine could inhibit the proliferation of K562 cells with inhibition rate of 69% after 72 hours matrine induction; number of K562 cells increased significantly in G1 phase, and number of apoptotic cell was 68% at 5th day of matrine treated cells; the typical morphology of apoptotic cells of K562 cells was observed by electron microscopy; BCL-6 was increased and PCNA was decreased in K562 cells induced by matrine. CONCLUSION: Matrine inhibited the proliferation and induced the apoptosis of K562 cells significantly, the mechanism of which might be related to cell cycle arrest and the change of PCNA and BCL-6.