| 三氯醋酸-丙酮沉淀法用于骨组织蛋白的提取 |
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| 引用本文： | 崔永锋,杨贞,朱宝华,肖鲁伟,童培建.三氯醋酸-丙酮沉淀法用于骨组织蛋白的提取[J].中国医学科学院学报,2011,33(2). |
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| 作者姓名： | 崔永锋 杨贞 朱宝华 肖鲁伟 童培建 |
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| 作者单位： | 1. 杭州市萧山区第一人民医院骨科,杭州,311200
2. 浙江中医药大学骨伤科研究所,杭州,310053 |
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| 摘 要： | 目的 探讨三氯醋酸-丙酮沉淀法提取骨组织蛋白的可行性和效率.方法 采用盐酸脱钙法和三氯醋酸-丙酮沉淀法,分别用于骨组织蛋白的提取,并对两种方法的提取效率进行对比.结果 三氯醋酸-丙酮沉淀法可获得较高的骨蛋白提取率,与盐酸脱钙法相比,分子条带分布范围相近,且不受骨髓造血组织影响.结论 三氯醋酸-丙酮沉淀法可以用于骨组织蛋白质组的研究.
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| 关 键 词： | 三氯醋酸-丙酮沉淀法 骨组织 蛋白提取 |
Application of Trichioroacetic Acid-Acetone Precipitation Method for Protein Extraction in Bone Tissue |
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| CUI Yong-feng,YANG Zhen,ZHU Bao-hua,XIAO Lu-wei,TONG Pei-jian.Application of Trichioroacetic Acid-Acetone Precipitation Method for Protein Extraction in Bone Tissue[J].Acta Academiae Medicinae Sinicae,2011,33(2). |
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| Authors: | CUI Yong-feng YANG Zhen ZHU Bao-hua XIAO Lu-wei TONG Pei-jian |
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| Institution: | CUI Yong-feng1,YANG Zhen2,ZHU Bao-hua1,XIAO Lu-wei2,TONG Pei-jian21Department of Orthopedics,the First People's Hospital of Xiaoshan District,Hangzhou 311200,China2Institute of Orthopedics,Zhejiang University of Traditional Chinese Medicine,Hangzhou 310053,China |
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| Abstract: | Objective To explore the feasibility and efficiency of extracting protein from bone tissue using trichloroacetic acid(TCA)-acetone precipitation method.Methods Hydrochloric acid(HCL) decalcification method and TCA-acetone precipitation method were separately used for bone protein extraction.The efficiencies of these two methods were compared.Results TCA-acetone precipitation method had significantly higher extraction efficiency.Compared with HCL decalcification method,it had less pollution from bone marrow ... |
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| Keywords: | trichloroacetic acid-acetone precipitation method bone tissue protein extraction |
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