细胞免疫反应性炎症性肠病动物模型的建立

目的 探讨炎症性肠病（ＩＢＤ）的发病机制,建立评价肠道炎症严重度和监测炎症活动性的指标。方法 用三硝基苯磺酸（ＴＮＢＳ）３０ｍｇ／５０％乙醇０．２５ｍｌ制作实验性ＩＢＤ的动物模型（ＴＮＢＳ／乙酰模型）,用１０ｍｇＴＮＢＳ诱导了复发性ＩＢＤ模型,对模型进行了大体标本和光学显微镜观察,并检测了病变组织的髓过氧化物酶（ＭＰＯ）和超氧化物歧化酶（ＳＯＤ）活性。结果 ＴＮＢＳ３０ｍｇ／５０％乙醇０．２５ｍｌ

The establishment of TNBS-induced experimental colitis

OBJECTIVE: To explore the pathogenesis of inflammatory bowel disease (IBD) and to establish some indices monitoring the activity and severity of IBD. METHODS: A rat model of experimental colitis was induced by administration of the hapten 2, 4, 6-trinitrobenzenesulfonic acid (TNBS, 30 mg) in 50% ethanol 0.25 ml as the "barrier breaker". And it then recurs only after 10 mg TNBS administration. Inflammation was assessed by gross appearance using a grading scale and by histology. Myeloperoxidase(MPO) and superioxide dismutase(SOD) activities were also measured to evaluate the severity of inflammation. RESULTS: An acute inflammation with ulcers and neutrophil infiltration developed that evolved into a chronic inflammation with luminol narrowing and abundant fibrous connective tissue hypertrophy at 21 days. Crypt abcess were observed in some rats killed in 1 week. MPO activity was significantly elevated, while SOD decreased, in mucosa from all treated groups at all time intervals when macroscopic and microscopic mucosal injury was evident. CONCLUSIONS: These results indicate that this model affords an opportunity to study the pathogenesis of colonic inflammatory disease and may be used to evaluate new treatments potentially applicable to IBD in humans. In addition, MPO and SOD activities could be regarded as two markers which may evaluate the severity of colonic inflammation.