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人乳头瘤病毒高低危基因型感染子宫颈组织的比较蛋白质组学分析及其差异
作者姓名:Qi SZ  Zhang GC  Zhang JP  Zeng XS  Cao YH  Zhong MY  Tao XH  Liu TY  Wang QQ  Yang RG
作者单位:1. 中国医学科学院,北京协和医学院,皮肤病研究所性病科,南京,210042
2. 中国科学院,武汉病毒研究所病毒学国家重点实验室,武汉,430071
基金项目:病毒学国家重点实验室开放研究基金;中国医学科学院皮肤病研究所重点科研项目
摘    要:目的观察人乳头瘤病毒(HPV)感染子宫颈组织后蛋白质组表达情况,探讨高、低危基因型感染子宫颈组织后表达的差异及意义。方法采用比较蛋白组学研究技术,对HPV高危基因型感染的子宫颈上皮内瘤变、低危基因型感染的子宫颈尖锐湿疣和正常对照的子宫颈上皮组织进行两两比较,筛选差异表达蛋白质,并对差异蛋白质点进行质谱分析。结果对3组26个差异蛋白质点的分析共获得了22张肽质量指纹图,搜索后得到18个与其相匹配的蛋白质点。通过查询NCBInr数据库获得初步鉴定的18个蛋白质功能信息,主要与细胞代谢、信号传导、细胞分泌、细胞骨架构建及细胞增殖、分化与凋亡等相关。结论高、低危基因型HPV感染子宫颈组织后蛋白质组表达存在明显差异。

关 键 词:人乳头瘤病毒  子宫颈上皮内瘤变  尖锐湿疣  双向凝胶电泳  质谱分析  比较蛋白质组学
文章编号:1000-503X(2007)05-0597-06
修稿时间:2007-07-10

Comparative proteome analysis of human papillomavirus-infected cervical specimens and the difference between the high- and low-risk genotypes of human papillomavirus
Qi SZ,Zhang GC,Zhang JP,Zeng XS,Cao YH,Zhong MY,Tao XH,Liu TY,Wang QQ,Yang RG.Comparative proteome analysis of human papillomavirus-infected cervical specimens and the difference between the high- and low-risk genotypes of human papillomavirus[J].Acta Academiae Medicinae Sinicae,2007,29(5):597-602.
Authors:Qi Shu-zhen  Zhang Guo-cheng  Zhang Jin-ping  Zeng Xue-si  Cao Yuan-hua  Zhong Ming-ying  Tao Xiao-hua  Liu Tong-yun  Wang Qian-qiu  Yang Rong-ge
Institution:1 Department of Venereology, Institute of Dermatology, CAMS and PUMC, Nanjing 210042, China; 2 State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
Abstract:Objective To perform an comparative proteome analysis of human papillomavirus-infected cervical specimens and to investigate different expressions between high-and low-risk genotypes.Methods The cervical specimens were divided into two groups(cervical intraepithelial neoplasia group and condyloma acuminatum group) according to their genotypes.Using comparative proteome technology,high-risk human papillomavirus-infected cervical intraepithelial neoplasia,low-risk human papillomavirus-infected condyloma acuminatum,and normal cervical intraepithelial tissue were compared.The differential expression protein spots were identified by mass spectrometry.Results Totally 26 differential spots were selected and analyzed,and 22 peptide mass fingerprints(PMF) maps were obtained by MALDI-TOF-MS.Eighteen proteins were preliminarily identified after searching the NCBInr database.The function information of these 18 proteins mainly involved cell metabolism,signal transduction,cell secretion,cell cytoskeleton construction,cell proliferation,and apoptosis.Conclusion The proteomic expressions after the cervical infection of high-or low-risk genotype of human papillomavirus are obviously different.
Keywords:human papillomavirus  cervical intraepithelial neoplasia  condyloma acuminatum  two-dimensional gel electrophoresis  mass spectrometry  comparative proteome
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