聚氨酯基纳米碳复合材料表面的血液相容性研究

目的研究聚氨酯基纳米碳复合材料表面的血液相容性。方法用溶液共混方法将经过表面处理后的纳米碳分散到聚氨酯体系中,制成聚氨酯/纳米碳复合薄膜。通过血小板荧光标记人全血灌注实验和羊全血体外循环实验,观察和测定血小板在材料表面的粘附以及血液中血红蛋白浓度、纤维蛋白原浓度的变化,并探讨纳米碳对聚氨酯抗凝血性能的影响。结果(1)全血灌注实验中,血小板的粘附数量从低到高顺序为:纤维蛋白原预处理的聚氨酯/纳米碳、聚氨酯/纳米碳、纤维蛋白原预处理的聚氨酯、聚氨酯;聚氨酯/纳米碳复合材料表面的血小板粘附数量显著减少。(2)体外循环4h后,与聚氨酯/纳米碳复合材料表面接触的血液中,血红蛋白浓度、纤维蛋白原浓度的变化均相对减小。结论将纳米碳引入到聚氨酯体系中,可以改善聚氨酯材料的血液相容性,是开发抗凝血材料的一个新途径。

Studies on the blood compatibility of the polyurethane/nano-sized carbon composite

Objective To investigate the effects of nano - sized carbon as a dispersed phase on blood compati - bility of polyurethanes. Methods A novel nanoscale polymeric composite film was prepared by dis - persing nano - sized carbon fiber ( vapor growth carbon fiber ) into the polyurethane solutions.The surface blood compatibilities of the composites were analyzed and evaluated through platelet adhesion measu - rement using epifluorescent video microscopy and the variation of fibrinogen and free hemoglobin concentration in the blood contacting the composite respectively. Results It was showed that the platelet adhesions were highly suppressed on the composite surfaces pre - adsorbed or non - pre - adsorbed with fibrinogen.The changes of the concentration for both free hemoglobin and fibrinogen in the blood contacting the composite surface in the circulations were less than the ones contacting the reference surface. Conclusions Introducing nano - sized carbon into the polyurethane matrix showed an improvement of antithrombogenicity for the polyurethane materials.It might be a new promising way to develop biomaterials with good blood compatibility.