组织工程中胶原基纳米骨复合材料的研制

目的：依据仿生原理制备纳米骨框架材料。方法：以胶原分子为模板,调制钙磷盐在液相中沉积其上,得到矿化胶原基复合材料,并采用液相分离法与少量聚乳酸复合进一步制备成为三维多孔框架材料。分离成骨细胞并在三维框架材料上培养,用x-ray、衍射扫描电镜、透射电镜、荧光显微镜、激光共聚焦显微镜进行观察和分析。结果：胶原基纳米晶钙磷盐复合材料的晶粒度较低,晶体极为细小,与天然骨类似;材料为多孔状,孔隙率较高;内部为典型的纤维束状结构,该纤维束有HA晶体的衍射图样,且具有002]择优取向;成骨细胞可在其上贴附、生长和繁殖。结论：仿生制备的三维胶原基纳米骨框架材料,无论从结构还是性能上,都是骨组织工程中的优选材料之一。

Mineralized collagen based composite for bone tissue engineering

To construct a mineralized collagen based composite by biomimetic synthesis for bone tissue engineering.Methods Using the molecular collagen as the template,the calcium phosphate is deposited on it to produce a mineralized collagen based composite,then is combined with minute amount of poly lactic acid(PLA),the three-dimensional scaffold composite is prepared by liquid phase separation.Using osteoblast culture technique,the biocompatibility of this biomaterial in vitro is detected by x-ray diffraction,SEM,TEM,fluoroscopy and CLSM.Results Both degree and the size of crystals in the composite are low,which are similar to that of nature bone.It possesses porous structure and the porosity of the composite is high.The typical fibrillar microstructure is self-assembled of the collagen and the nano-crystal hydroxyapatite(HA)in the composite,moreover,the x-ray diffraction graphic of HA crystal shows the-oriented.Conclusions The biomimetic three-dimensional composite can serve as one of the optimal scaffold material for bone tissue engineering both on structure and on property.