| 趋化素样因子（CKLF1）对骨髓细胞增殖活性的研究 |
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| 作者姓名： | Han WL Rui M Zhang YM Chen YY Zhong YC Di CH Song QS Ma DL |
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| 作者单位： | 北京大学基础医学院免疫系, |
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| 基金项目： | 国家高技术研究发展计划863课题(102-08-07-04-99)资助
Supported by the National High Technology Research and Development Program(102-08-07-04-99); |
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| 摘 要： | 目的 研究趋化素样因子1（CKLF1）对造血功能的调节作用。方法 利用MTT法,检测CKLF1真核细胞表达载体转染上清在液体培养基中,对人低密度骨髓细胞和小鼠骨髓细胞的增殖调控作用;并在兰固体培养基中,观察其对人低密度骨髓细胞集落形成的刺激作用。结果 COS－7细胞表达的CKLF1能明显促进人和小鼠骨髓细胞的增殖,并能促进人骨髓细胞的集落形成,与GM－CSF有协同刺激作用。结论 CKLF1能促进人和小鼠骨髓造血干／祖细胞的增殖和分化。
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| 关 键 词： | 趋化素样因子1 造血干细胞 集落形成 骨髓细胞增殖 |
| 修稿时间： | 2000年12月12 |
Stimulatory effect of chemokine-like factor 1 (CKLF1) on the growth of bone marrow cells |
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| Han WL,Rui M,Zhang YM,Chen YY,Zhong YC,Di CH,Song QS,Ma DL.Stimulatory effect of chemokine-like factor 1 (CKLF1) on the growth of bone marrow cells[J].Acta Academiae Medicinae Sinicae,2001,23(2):119-122. |
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| Authors: | Han W L Rui M Zhang Y M Chen Y Y Zhong Y C Di C H Song Q S Ma D L |
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| Institution: | Department of Immunology, Basic Medical School, Peking University, Beijing 100083, China. |
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| Abstract: | OBJECTIVE: To investigate the activity of CKLF1 on the proliferation and differentiation of bone marrow cells. METHODS: Human low density bone marrow cells and mouse bone marrow cells were plated in 96-well microplate and supernatants from transfected COS-7 cell culture were added. The cell proliferation was assayed by MTT method after 5 days incubation. The enhancing effect of CKLF1 on the colony formation of human hematopoietic progenitor cells was identified in semi-solid culture. RESULTS: CKLF1 has obvious enhancing effect on both human and mouse bone marrow cells, it can stimulate the colony formation of human hematopoietic stem cells and has synergistic action with GM-CSF. CONCLUSION: CKLF1 can promote the proliferation and differentiation of bone marrow cells. |
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| Keywords: | hemokine-like factor 1 hematopoietic stem cells granulocyte-macrophage colony stimulating factor |
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