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C57BL/6J小鼠慢性束缚应激抑郁症模型的建立
引用本文:孙楠楠,沙龙泽,许琪.C57BL/6J小鼠慢性束缚应激抑郁症模型的建立[J].中国医学科学院学报,2015,37(2):8-11.
作者姓名:孙楠楠  沙龙泽  许琪
作者单位:中国医学科学院基础医学研究所 北京协和医学院基础学院 生物化学与分子生物学系医学分子生物学国家重点实验室,北京 100005
基金项目:国家自然科学基金(31222031)、中央高校基本科研业务费专项资金(2012S05)和协和青年科研基金(2012J09)
摘    要:目的 通过慢性束缚应激(CRS)建立C57BL/6J小鼠抑郁症模型。方法 C57BL/6J小鼠经体重、糖水偏好、旷场实验初筛后从中选择20只小鼠并随机分为正常对照(NC)组和CRS组2组,每组10只。对CRS组小鼠进行连续21 d,每天4 h的束缚刺激,并最终进行糖水偏好检测和强迫游泳检测。结果 CRS组小鼠在水中不动的时间为(131.70±21.65)s,明显长于NC组的(68.88±8.43)s(P=0.0304)。CRS组小鼠0~24 h和0~48 h的糖水偏好百分比分别为(66.21±3.24)%和(73.25±1.50)%,均明显低于NC组的(79.46±3.85)%(P=0.0196)和(80.20±2.26)%(P=0.0248)。结论 通过慢性束缚应激成功建立C57BL/6J小鼠抑郁症模型。

关 键 词:抑郁症  慢性束缚应激  C57BL/6J小鼠  行为学检测
收稿时间:2014-09-30

Establishment of a Chronic Restraint Stress Model of Depression with C57BL/6J Mice
SUN Nan-nan,SHA Long-ze,XU Qi.Establishment of a Chronic Restraint Stress Model of Depression with C57BL/6J Mice[J].Acta Academiae Medicinae Sinicae,2015,37(2):8-11.
Authors:SUN Nan-nan  SHA Long-ze  XU Qi
Institution:State Key Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China
Abstract:Objective To establish a chronic restraint stress(CRS)model of depression,using C57BL/6J mice. Methods Totally 20 C57BL/6J mice were screened by weight,sucrose preference,and the results of open-field test. Then,they were equally randomized into two groups:normal control(NC)group and CRS group. Mice in the CRS group were under restraint 4 hours a day and their behavioral changes were evaluated after 21 days. Results The immobility time was significantly longer in CRS mice(131.70±21.65) s] compared with controls(68.88±8.43) s](P=0.0304). CRS mice showed a significant decrease in sucrose preference during the time 0-24 h (66.21±3.24)% vs.(79.46±3.85)%,P=0.0196] and 0-48 h (73.25±1.50)% vs.(80.20±2.26)%,P=0.0248]compared with controls. Conclusion The C57BL/6J mice CRS models of depression were successfully established.
Keywords:depression  chronic restraint stress  C57BL/6J mice  behavioral tests
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