环氧合酶-2-1195G/A和锰超氧化物歧化酶9Ala/Val基因多态性与高脂饮食的交互作用及其与溃疡性结肠炎的关系

目的 探讨环氧合酶-2-1195G/A(COX-2-1195G/A)和锰超氧化物歧化酶9Ala/Val(MnSOD9Ala/Val)基因多态性与高脂饮食的交互作用及其与溃疡性结肠炎(UC)的关系。方法 采用病例-对照研究的方法,以750例UC患者及750例健康对照者的外周血白细胞为样本,采用聚合酶链反应(PCR)技术分析COX-2-1195G/A和MnSOD9Ala/Val基因多态性。结果 UC组和对照组COX-2-1195G/A(A/A)基因型的分布频率分别为49.07%和21.20%,MnSOD9Ala/Val(V/V)基因型的分布频率分别为50.13%和22.40%,差异均有统计学意义(P均＜0.01)。COX-2-1195G/A(A/A)基因型(OR=3.5808,95%CI=1.8062～5.3478)和MnSOD9Ala/Val(V/V)基因型(OR=3.4828,95%CI=1.9137～5.5496)者患UC的风险均显著增加。基因突变的协同分析结果显示,COX-2-1195G/A(A/A)/MnSOD9Ala/Val(V/V)基因型者在UC组和对照组中的分布频率分别为40.67%和8.40%,差异有统计学意义(P＜0.01),COX-2-1195G/A(A/A)/MnSOD9Ala/Val(V/V)基因型者患UC的风险显著增加(OR=7.5655,95%CI=4.1849～11.2037)。UC组高脂饮食率显著高于对照组(49.73%比20.13%,P＜0.01),高脂饮食与COX-2-1195G/A(A/A)(γ=11.81821)和 MnSOD9Ala/Val(V/V)(γ=9.0107)基因型均有交互作用。结论 COX-2-1195G/A(A/A)和MnSOD9Ala/Val(V/V)基因型及高脂饮食是UC的易患因素,基因多态性与高脂饮食的交互作用增加了UC的发病风险。

Interaction between the Polymorphisms of Cyclooxygenase-2-1195G/A,MnSOD9Ala/Val Genes and the High-fat Diets and Its Correlation with Ulcerative Colitis

Objective To investigate the interaction of the polymorphisms of cyclooxygenase-2-1195G/A(COX-2-1195G/A)and manganese superoxide dismutase 9Ala/Val(MnSOD9Ala/Val)genes and the high-fat diets and its potential correlation with ulcerative colitis(UC). Methods The genetic polymorphisms of COX-2-1195G/A and MnSOD9Ala/Val were analyzed by polymorphism-polymerase chain reaction(PCR)in peripheral blood leukocytes obtained from 750 UC patients(UC group)and 750 healthy subjects(control group). Results The frequencies of COX-2-1195G/A(A/A)and MnSOD9Ala/Val(V/V)were 49.07% and 50.13% in UC group and 21.20% and 22.40% in control group,respectively(P＜0.01). The risk of UC significantly increased in subjects with COX-2-1195G/A(A/A)genotype(OR=3.5808,95%CI=1.8062-5.3478)and in those with MnSOD9Ala/Val(V/V)genotype(OR=3.4828,95%CI=1.9137-5.5496). Pooled analysis of the polymorphisms showed that distribution frequency of COX-2-1195G/A(A/A)/MnSOD9Ala/Val(V/V)was 40.67% in UC group and 8.40% in control group(P＜0.01). Subjects with COX-2-1195G/A(A/A)/MnSOD9Ala/Val(V/V)had a significantly higher risk of UC(OR=7.5655,95%CI=4.1849-11.2037). The rate of high-fat diets was significantly higher in the UC group than in the control group(49.73 vs.20.13%,P＜0.01),and statistic analysis suggested an interaction between high-fat diet and COX-2-1195G/A(A/A)(γ=11.81821)and MnSOD9Ala/Val(V/V)(γ=9.0107),which increase risk of UC. Conclusions COX-2-1195G/A(A/A),MnSOD9Ala/Val(V/V),and high-fat diet are the risk factors of UC. The interaction between the genetic polymorphisms of COX-2-1195G/A and MnSOD9Ala/Val and the high-fat diet increases the risk of UC.