| 抗血管内皮生长因子165抗体轻重链可变区基因的克隆与？… |
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| 引用本文： | 冉宇靓,杨治华.抗血管内皮生长因子165抗体轻重链可变区基因的克隆与？…[J].中国医学科学院学报,1998,20(6):423-427. |
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| 作者姓名： | 冉宇靓 杨治华 |
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| 作者单位： | 中国医学科学院中国协和医科大学肿瘤研究所，北京 100021 |
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| 摘 要： | 获得抗血管内皮生长因子１６５抗体的轻重链可变区基因。方法从分泌具有中和抗血管皮生长因子１６５活性的单抗杂交瘤细胞株Ｖｍｄ１１中提取总ＲＮＡ，经ＲＴ－ＰＣＲ扩增并克隆该单抗的轻，重链可变区基因并进行序列分析。
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| 关 键 词： | VEGF165 抗体 RT－PR 克隆 可变区基因 序列分析 |
| 修稿时间： | 1998年3月12日 |
Cloning and Sequencing of the VL and VH Genes from the Anti-vascular Endothelial Growth Factor McAb Hybridoma Cell Line |
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| Y Ran,Z Yang,G Wang,Z Dong.Cloning and Sequencing of the VL and VH Genes from the Anti-vascular Endothelial Growth Factor McAb Hybridoma Cell Line[J].Acta Academiae Medicinae Sinicae,1998,20(6):423-427. |
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| Authors: | Y Ran Z Yang G Wang Z Dong |
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| Institution: | Cancer Institute, CAMS and PUMC, Beijing 100021. |
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| Abstract: | OBJECTIVE: The light chain variable region (VL) and heavy chain variable region (VH) genes from the anti-VEGF165 McAb hybridoma cell line were cloned for construction of it's chimeric antibody. METHODS: The VL and VH genes were amplified from the total RNA of the VmD11 hybridoma with RT-PCR and cloned into vectors, and then analyzed by sequencing. RESULTS: The 320 bp VL gene belong to mouse kappa light chain subgroup V, the 354 bp VH gene belong to mouse heavy chains subgroup I (B). CONCLUSIONS: The nucleotide sequence analysis indicated that the cloned genes coded the light and heavy chain variable region of mouse respectively. |
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