| 人浆细胞瘤与小鼠网织红细胞异种杂交的恶性调控研究 |
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| 引用本文： | 陈克铨,张乐英,唐瑾,汪兆琦,史顺娣,薛社普.人浆细胞瘤与小鼠网织红细胞异种杂交的恶性调控研究[J].中国医学科学院学报,1988(1). |
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| 作者姓名： | 陈克铨 张乐英 唐瑾 汪兆琦 史顺娣 薛社普 |
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| 作者单位： | 中国医学科学院基础医学研究所，中国医学科学院基础医学研究所，中国医学科学院基础医学研究所，中国医学科学院基础医学研究所，中国医学科学院实验动物研究所，中国医学科学院基础医学研究所 北京，北京，北京，北京，北京 |
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| 摘 要： | 用人浆细胞瘤细胞与小鼠网织红细胞异种杂交获得成功。杂交细胞可以传代,出现下列明显的肿瘤细胞去恶性化特征:①生长速度及~H-TdR并合率明显下降;②出现红细胞特异的遗传标记——血红蛋白;③出现慢型迁移率的葡萄糖-6-磷酸异构酶Gpi;④在软琼脂培养基中不形成细胞集落;⑤异种移植至裸鼠后丧失发生肿瘤的能力。表明红细胞胞质体杂交模型可以用作研究细胞恶性调控的实验系统。对调控肿瘤细胞恶性表型的可能机理进行了讨论。
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| 关 键 词： | 人浆细胞瘤 网织红细胞 胞质杂交 血红蛋白 葡萄糖-6-磷酸 异构酶 |
Studies on Malignancy Regulation by Heterologous Cybridization between Reticulocytes and Human Plasmocytoma Cell Line (HMY-2) |
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| Chen Kequan,.Studies on Malignancy Regulation by Heterologous Cybridization between Reticulocytes and Human Plasmocytoma Cell Line (HMY-2)[J].Acta Academiae Medicinae Sinicae,1988(1). |
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| Authors: | Chen Kequan |
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| Abstract: | The present paper reported a heterologous cybrid cell line established in our laboratory by fusion of mouse reliculocytes with human plasmocy-toma cells. These cybrid cells could be continuously cultured and passaged without loss of characteristics of decancerization : 1. Obvious decrease of growth rate and incorporation rate of 3H-thymidine in nuclei; 2. Expression of specific genetic marker of reticulocyte hemoglobin; 3. Expression of glucose-6-phosphate isomerase with low mobility rate; 4. Loss of growth ability in soft-agar medium; and 5. Nontumorigenicity under heterotransplantation in to nude mice. These results provided further evidence, confirming this erythroid cy-brid model being useful in studies on malignancy regulation. The possible mechanism of cybrid model in regulation of tumor malignant phenotype was discussed. |
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| Keywords: | human plasmocytoma (HMY-2) reticulocyte cybridizationhemoglobin glucose-6-phosphate isomerase |
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