| 结缔组织生长因子基因的克隆 |
| |
| 作者姓名： | Li Q Zhao H Wang J Fu J Zhao S Jiang L |
| |
| 作者单位： | 中国医学科学院!昆明650118,中国协和医科大学医学生物研究所病毒免疫室,中国医学科学院!昆明650118,中国协和医科大学医学生物研究所病毒免疫室,中国医学科学院!昆明650118,中国协和医科大学医学生物研究所病毒免疫室,中国医学科学院!昆明650118,中国协和医科大学医学生物研究 |
| |
| 基金项目： | 云南省科技攻关项目(95C17-1)资助 Supported
by the Yunnan Provi ncial |
| |
| 摘 要： | 克隆细胞即刻早期基因反应产物人结缔组织生长因子基因,对分析细胞基因反应及用国修复研究的理论和应用研究均具有较大意义。方法使用组织培养的方法,建立了人脐静脉内皮细胞系在适当刺激后,提取ｍＲＮＡ,应用ＲＴ－ＰＣＲ方法,分段克隆人结缔组织生长因子基因,并经无义突变形成酶切位点使之连接。
|
| 关 键 词： | 结缔组织 生长因子 CTGF基因 克隆 |
| 修稿时间： | 1998-04-20 |
Investigation of connective tissue growth factor's gene cloning |
| |
| Li Q,Zhao H,Wang J,Fu J,Zhao S,Jiang L.Investigation of connective tissue growth factor's gene cloning[J].Acta Academiae Medicinae Sinicae,2000,22(1):48-51. |
| |
| Authors: | Li Q Zhao H Wang J Fu J Zhao S Jiang L |
| |
| Institution: | Department of Viral and Immunology, Institute of Medical Biology, CAMS, PUMC, Kunming 650118, China. glimbcam@km.ym.cn |
| |
| Abstract: | Objective The human connective tissue growth factor(CTGF),as the responsive product of immediate early gene,was cloned for the investigation of cellular primary gene responsed and healing repairing. Methods RT PCR was performed with the mRNA of HUVE cell stimulated,the three clones of CTGF,via mutational modified,were ligated to a complete gene that identified with sequencing. Results The sequencing indicated that this CTGF gene contains complete coding region and the 3' noncoding region which is difference with the reported sequence. Conclusion A new human CTGF gene was cloned. |
| |
| Keywords: | connective tissue growth factor gene clone |
| 本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录！ |
|
