促卵泡激素抑制顺铂诱导的卵巢癌细胞凋亡

目的 探讨促卵泡激素(FSH)对顺铂诱导卵巢癌细胞凋亡的影响及其机制。方法 应用DNA Fragmentation梯带电泳分析，TUNEL荧光原位凋亡检测法测定对FSH敏感的卵巢癌细胞系OVCAR3(OVCAR3-FSHR)细胞凋亡状态，应用Caspase-3活性分析法检测Caspase-3活性，RT-PCR检测凋亡相关基因survivin mRNA表达，Western blot检测其Survivin及bcl-2蛋白表达。结果 不论是DNA梯带电泳还是TUNEL荧光原位凋亡检测都表明促卵泡激素能抑制顺铂诱导的OVCAR3-FSHR细胞的凋亡。Caspase-3活性分析表明促卵泡激素能降低顾铂引起的Caspase-3活性增加。半定量RT-PCR结果表明FSH能增加survivin mRNA表达，Western blot结果表明FSH能增加Survivin蛋白表达而对bcl-2表达无影响。结论 促卵泡激素能够抑制顺铂诱导卵巢癌细胞凋亡，其作用机剖可能与促进Survivin表达及降低Caspase-3活性有关。

Follicle stimulating hormone inhibits cisplatin induced apoptosis in ovarian cancer cells

OBJECTIVE: To investigate whether the follicle stimulating hormone (FSH) can inhibit apoptosis in ovarian cancer cells induced by cisplatin (DDP) and its possible mechinism. METHODS: DNA fragmentation assay, (TdT-mediated dUTP nick end labling TUNEL), Western blot were used to analyze the changes in expression levels of Survivin and bcl-2 protein. The relative activity of caspase-3 was also determined. RESULTS: 200 mIU/ml FSH could regulate down the percentage of apoptotic cells and DNA fragmentation induced by 5.0 micrograms/ml cisplatin, while 200 mIU/ml FSH increased Survivin protein expression but could't influence the expression of bcl-2 protein. CONCLUSION: FSH can inhibit ovarian cancer cells apoptosis induced by cisplatin. The possible mechinism is up-regulation of Survivin expression and down-regulation of caspase activity.