鬼针草总黄酮有效部位提取工艺的优化

目的建立总黄酮的含量测定方法,优选鬼针草中黄酮类成分的提取工艺。方法以芦丁为对照物,用紫外分光光度法建立总黄酮的含量测定方法。以提取液中总黄酮的量为指标,用正交设计法对影响提取效果的因素如乙醇浓度、乙醇用量、提取次数和提取时间进行考察,优化提取工艺。结果芦丁在0.004 7-0.023 5 mg/ml范围内线性关系良好,吸收度(A)和浓度(c,mg/ml)的线性方程为A=28.723c 0.043 4,r=0.999 6(n=5),加样回收率为98.58%,RSD=1.1%;理化鉴别表明乙醇溶液可将鬼针草中黄酮类成分有效地提取出来,总黄酮的最佳提取工艺为12倍量80%乙醇,连续提取3次,每次1.0 h。结论本实验为鬼针草中总黄酮的检测提供了定性、定量的检测方法。所优选出的鬼针草中总黄酮类成分的最佳提取工艺具有效率高和重现性好等特点。

Optimization of the extraction conditions for flavonoid constituents in spanishneedles herb

Objective To optimize the extraction conditions for flavonoid constituents of spanishneedles herb,and to establish a method to quantitatively determine the total flavonoids from the extracts of spanishneedles herb.Methods The total flavonoids in spanishneedles extracts was determined by UV-spectrophotometry.The concentration of ethanol,quantity of ethanol,extraction times and time of extraction were investigated by orthogonal test with the quantity of total flavonoids as a target index.Results The total flavonoids in spanishneedles herb were extracted effectively by ethanol.Linear range of the rutin concentration was 0.004 7-0.023 5 mg/ml,and regression equation was A=28.723c 0.043 4,r=0.999 6(n=5).RSD was less than 5%.The mean recovery was 98.58%.The optimized extraction conditions were 12 times as much as the volume of 80% ethanol for three csucessive extraction and 1.0 h per time.Conclusion The method could qualitatively and quantitatively determine the total flavonoid in spanishneedles herb.The optimized extraction method is stable and efficient.