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| 改良的降落PCR扩增中国地鼠目的基因 | |
| 引用本文： | 宋国华,庞文彪,张锐虎,刘田福,岳文斌.改良的降落PCR扩增中国地鼠目的基因[J].山西医科大学学报,2008,39(10). |
| 作者姓名： | 宋国华 庞文彪 张锐虎 刘田福 岳文斌 |
| 作者单位： | 1. 山西医科大学实验动物中心,太原,030001;山西农业大学动物科技学院 2. 山西医科大学实验动物中心,太原,030001 3. 山西农业大学动物科技学院 |
| 基金项目： | 山西省实验动物专项基金资助项目(2005K01) |
| 摘 要： | 目的优化PCR程序，尝试用降落PCR扩增中国地鼠目的基因。方法设计了4对引物，用普通PCR和改良的降落PCR扩增中国地鼠COX1、COX2、NADH5、NADH6部分基因。结果普通PCR只有1对引物扩增出特异性条带，而降落PCR4对引物都扩增出特异性条带，并与中国地鼠目的基因大小一致。结论降落PCR省略了常规PCR中摸索最适退火温度的过程，对中国地鼠一些Tm值相近的基因可以使用降落PCR温度程序扩增，是一种行之有效的PCR方法。 |
| 关 键 词： | 中国地鼠 降落PCR 退火温度 PCR特异性 |
Modified touchdown PCR as an approach to amplify target gene of Cricetulus grisens |
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| SONG Guo-hua,PANG Wen-biao,ZHANG Rui-hu,LIU Tian-fu,YUE Wen-bin.Modified touchdown PCR as an approach to amplify target gene of Cricetulus grisens[J].Journal of Shanxi Medical University,2008,39(10). | |
| Authors: | SONG Guo-hua PANG Wen-biao ZHANG Rui-hu LIU Tian-fu YUE Wen-bin |
| Institution: | 1Laboratory Animal Center;Shanxi Medical University;Taiyuan 030001;China;2College of Animal Science and Technology;Shanxi Agricultural University |
| Abstract: | Objective To amplify target genes of Cricetulus griseus by modified touchdown PCR(TD-PCR).Methods Common PCR and TD-PCR were used to amplify genes of Cricetulus griseus with 4 pairs primers designed based on conserved gene coding regions of consensus sequence COX1,COX2,NADH5,NADH6,respectively.Results Only one fragment was amplified by common PCR,but 4 target fragments were amplified by TD-PCR.Conclusion Compared with common PCR,TD-PCR need not to search best annealing temperature.And TD-PCR is a beneficial... |
| Keywords: | Cricetulus griseus touchdown PCR annealing temperature PCR specificity |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |