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三七提取液中三七总皂苷的分离纯化工艺研究
引用本文:谢茵,邢桂琴,刘秀芬.三七提取液中三七总皂苷的分离纯化工艺研究[J].山西医科大学学报,2006,37(6):613-615.
作者姓名:谢茵  邢桂琴  刘秀芬
作者单位:山西医科大学药学院药剂教研室,太原,030001
摘    要:目的研究影响分离纯化三七总皂苷的几个主要影响因素,确立三七总皂苷分离纯化工艺。方法在确立三七总皂苷测定方法的基础上,采用HPD-100型大孔吸附树脂,以每ml含0.4 g三七药材的提取液为上柱液,上柱量为1.5倍柱体积,并以1柱体积/h的速度通过树脂柱,采用大孔吸附树脂富集分离纯化三七总皂苷。结果用70%乙醇为洗脱剂,2-3倍柱体积/h的速度洗脱,用量为5-6倍柱体积时,三七总皂苷的洗脱率达98%以上,纯度为79.6%,精制度为241%。结论HPD-100大孔吸附树脂可用于富集、分离纯化三七总皂苷,效果较好。

关 键 词:三七总皂苷  HPD-100大孔吸附树脂  洗脱率  精制度
文章编号:1007-6611(2006)06-0613-03
收稿时间:2006-01-05
修稿时间:2006年1月5日

Study on the separation and purification of radix notoginseng saponin
XIE Yin,XING Gui-qin,LIU Xiu-fen.Study on the separation and purification of radix notoginseng saponin[J].Journal of Shanxi Medical University,2006,37(6):613-615.
Authors:XIE Yin  XING Gui-qin  LIU Xiu-fen
Institution:Dept of Pharrnaceutics, Pharmacy College, Shanxi Medical University, Taiyuan 030001, China
Abstract:Objective To optimize the major conditions for the separation and purification of radix notoginseng saponin. Methods On the basis of determining radix notoginseng saponin,HPD-100 macroreticular resin was used for enriching radix notoginseng saponin.The extraction of 0.4 g material/ml was adsorbed in 1.5 times of column volume at a speed of one column volume per hour. Results The extraction was eluted by 70% ethanol of 5-6 times with column volume at a flow rate of 2-3 folds column volume per hour.Elution ratio of radix notoginseng saponin was more than 98%,purity was 79.6%,polishing was 241%. Conclusion HPD-100 macroreticular resin can be used to enrich,separate and purify radix notoginsen saponin,and the effect is better.
Keywords:radix notoginseng saponin  HPD-100 macroreticular resin  elution ratio  polishing
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