湖州铁线莲乙醇提取物体外抗K562与MGC-803活性研究

目的]研究湖州铁线莲乙醇提取物体外抗肿瘤作用。方法]干燥湖州铁线莲经石油醚脱脂、乙醇回流得到初提物。采用MTT法观察经AB-8树脂静态吸附70%乙醇解析处理后湖州铁线莲乙醇提取物体外对人白血病K562细胞及人胃癌MGC-803细胞的增殖抑制作用。结果]在倒置显微镜下,湖州铁线莲处理组K562细胞形态不规则,MGC-803细胞体积缩小,部分细胞收缩变圆。两种细胞均生长缓慢,随着药物作用时间的延长,细胞的贴壁能力逐渐减弱,呈现出典型的凋亡形态学改变。MTT实验表明湖州铁线莲乙醇提取物对K562细胞及MGC-803细胞有增殖抑制作用,其作用68h的IC₅₀值分别为133.66mg·L^-1、317.87mg·L^-1。结论]体外实验中,湖州铁线莲乙醇提取物具有抗肿瘤作用,且呈现良好的浓度-效应依赖关系。

Study on the Anti-tumor Activty of Ethanol Extracts of Clemats huehouensh Tamura on K562 and MGC-803

Purpose] To observe the anti-tumor activity of the ethanol extracts of Clematis huchouensis Tamura in vitro. Method] To ob- tain preliminary extracts, dry C. huehouensis were degreased with petroleum ether and refluxed with ethanol. In vitro, the inhibi- tory effects of ethanol extracts of C. huchouensis by static adsorption treatment on proliferation of human leukemia K562 cells and gastric carcinoma MGC-803 cells were measured by MTT eolorimetrie assay. Result] The MTT test demonstrated that ethanol extracts of C. huchouensis inhibited proliferation of K562 cells and MGC-803 cells, and the IC50 values on them were 133.66mg/L and 317.87mg/L, respectively. After treatment with ethanol extracts from C. huchouensis, the K562 cells grew slowly, turned irregular and the adherent ability decreased. While MGC-803 cells also grew slowly, the volume of cells became small, some cells condensed round masses, the adherent ability decreased, presenting a typical apoptosis morphological change. Conclusion] In tumor inhibi- tory test, it was shown that the ethanol extracts of C, huchouensis may possess significantly inhibitory effect in vitro and the tu- mor inhibition rate was depending on drug concentration.