高血压病肝阳上亢证患者尿液样品的核磁共振谱代谢组学研究

目的]寻找高血压病肝阳上亢证患者可能的生物标记物,揭示其证候本质。方法]对2008年7月至2009年10月间山东省中医医院神经内科收治的9例高血压病肝阳上亢证患者与7例健康志愿者,用~1HNMR分析尿液成分,使用PCA方法进行模式识别,寻找标记物并做出相关代谢途径解释。结果]两组~1HNMR谱数据可以在得分图实现分类;与健康志愿者作比较,高血压病肝阳上亢患者机体相关代谢发生显著变化,体内氨基酸、葡萄糖代谢明显异于健康志愿者。结论]~1HNMR结合PCA模式识别的代谢组学方法具有研究复杂条件下机体病理生理变化的优势,为理解高血压病肝阳上亢证的证候本质和诊断提供了科学依据。

A Nuclear Magnetic Resonance Spectroscopic Metabonomics Analysis for Urine Samples of Hypertension Patients with Syndrome of Ascendant Hyperactivity of Liver Yang

Objective]Investigate the difference of urine composition spectrum between hypertension patients with syndrome of ascendant hyperactivity of liver yang and healthy volunteers to search possible biomarker.Reveal the nature of syndrome of ascendant hyperactivity of liver yang.Methods] Choose typical cases according to the clinic diagnostic criteria for hypertension patients with syndrome of ascendant hyperactivity of liver yang,and analyse the components in the urine of hypertension patients with syndrome of ascendant hyperactivity of liver yang and healthy volunteers by ’HNMR. Results]The data from the PCA analysis shows that:two sets of data can be classified in the score plot.There are significant differences existing between the two groups in terms of relative metabolism of body,especially for some amino acid metabolism and glucose metabolism.Conclusion]The study provided evidences for probing the essence of the hypertension of syndrome of ascendant hyperactivity of liver yang and researching the clinical diagnosis of such diseases.