| 红曲菌株的RAPD多态性分析 |
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| 引用本文： | 丁红梅,葛尔宁,王泽时.红曲菌株的RAPD多态性分析[J].浙江中医药大学学报,2007,31(5):613-614,617. |
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| 作者姓名： | 丁红梅 葛尔宁 王泽时 |
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| 作者单位： | 浙江中医药大学,杭州,310053 |
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| 基金项目： | 浙江省自然科学基金项目(Y204147)~~ |
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| 摘 要： | 目的]研究红曲分离株的遗传多样性和亲缘关系,为种质鉴定、保护和利用提供参考。方法]用随机引物对10个红曲菌株进行RAPD分析,从中筛选出63特征性好,多态性高的RAPD图谱,用NTSYS-PC-2.1软件进行同一性和差异性分析并根据遗传距离构建系统进化树。结果]红曲分离株间的同一性平均73.32%,表明它们的遗传背景具有很大的相似性;RAPD聚类结果与菌株的形态差异相关,表明RAPD分子标记进行菌株鉴别与传统形态分类鉴别结果基本一致,鉴别能力较强。结论]基于RAPD多态性的遗传分析能从分子水平上揭示红曲菌的遗传背景差异,为分类鉴定、种质起源和系统进化提供辅助手段和技术依据。
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| 关 键 词： | 红曲菌 RAPD分析 遗传同一性 系统进化树 |
| 文章编号： | 1005-5509（2007）05-0613-03 |
| 修稿时间： | 2007-01-12 |
Random Amplified Polymorphic DNA Analysis of Monascus Strains |
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| Ding Hongmei,Ge Erning,Wang Zeshi.Random Amplified Polymorphic DNA Analysis of Monascus Strains[J].Journal of Zhejiang University of Traditional Chinese Medicine,2007,31(5):613-614,617. |
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| Authors: | Ding Hongmei Ge Erning Wang Zeshi |
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| Abstract: | Objective] To study the genetic diversity and relative relationship of different Monascus trains to provide reference for classification and protection of germ plasm resources.Methods] Comparing the results of the random amplified polymorphic DNA(RAPD)fingerprints derived from 10 cultivated strains of Monascus,63 RAPD electrophoresis atlases were selected to calculate the genetic identity and divergence among them by NTSYS-PC-2.1 software.And then phylogenetic tree of Monascus strains was conceived accord... |
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| Keywords: | Monascus RAPD analysis genetic identity phylogenetic tree |
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