清肠愈溃膏抗大鼠溃疡性结肠炎作用研究

目的]观察清肠愈溃膏对溃疡性结肠炎(UC)实验大鼠的抗损伤治疗作用及其对超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量的影响。方法]将动物随机分为清肠愈溃膏组、SASP组、模型组、空白组,除空白组外其余3组动物分别用三硝基苯磺酸(TNBS)建立大鼠溃疡性结肠炎模型,连续用药2周后取结肠组织,镜下观察其结肠粘膜损伤及溃疡形成情况,并分别测定其SOD和MDA活性。结果]模型组大鼠结肠粘膜糜烂、溃疡、腺体破坏、炎细胞浸润,炎症评分与溃疡形成情况严重,SOD活力较空白组明显降低,MDA含量较空白组明显增高;其它各组分别有不同程度的炎症修复、溃疡愈合及SOD活性增高与MDA含量降低,其中尤其以清肠愈溃膏组最为明显。结论]TNBS急性损伤使大鼠结肠粘膜组织呈现炎症改变与溃疡形成,结肠组织SOD活性降低与MDA含量增高,其病理改变及氧化损伤情况符合溃疡性结肠炎病理变化;中药清肠愈溃膏能有效改善炎症病理及愈合溃疡,增强SOD活性、降低MDA含量,对实验性大鼠UC具有良好治疗作用。

Influence of Qingchangyukui Gel on Activity of SOD and Contents of MDA in Rats with Experimental Ulcerative Colitisbstract

Objective] To observe the curative effects and the influence of activity of Superoxide dismutase(SOD) and contents of Maleic dialdehyde(MDA) of Qingchangyukui Gel in Rats with Experimental Ulcerative Colitis. Method]Ulcerative Colitis model was established in Wistar rats by injecting trinitro-benzene-sulfonic acid (TNBS)into anus, and then treated with Qingchangyukui Gel and salisy-lazosulfa-pyridine(SASP) respectively. Isolating the colon and measuring the SOD and MDA after treating for two weeks. Results]Colon mucous membrane in the model group had serious erosion, ulcer, damaging of glandular organ, high inflammation grade; activity of SOD in model group was significantly lower than in normal group and contents of MDA increased. The other groups, especially in Qingchangyukui Gel group, had different levels of inflammation repairing, ulcer healing, increasing of SOD and reducing of MDA. Conclusion]TNBS can cause Colon mucous membrane inflammation and ulcer, reducing of SOD and increasing of MDA , which are the typical pathological change of Ulcerative Colitis; the Qingchangyukui Gel has the effects of increasing the activity of SOD and reducing contents of MDA, therefore it can effectively treat the UC rat.