蚯蚓组织成分对成纤维细胞增生作用的实验研究

通过蚯蚓组织成分对成纤维细胞增生作用的实验研究 ,探讨其对创伤愈合的作用机制。用醋酸盐缓冲液抽提蚯蚓组织成分 ,经硫酸铵分段盐析、阴离子交换层析、凝胶过滤层析等方法 ,分离提取到 1组蛋白质成分。采用MTT法分析各分离组分对小鼠成纤维细胞增生作用的影响。结果 :经分离提取促增生组分位于SephadexG1 0 0层析第 1峰 ,经SDS 聚丙烯酰胺凝胶电泳显示此促增生组分为 3条区带 ,相对分子质量范围 5 70 0 0、72 0 0 0、870 0 0。经实验证明蚯蚓组织中含有能够促进成纤维细胞增生的活性成分

Mitogenic Activity of Fibroblast in Earthworm

By means of experiment, the affect of the tissue components purified from earthworm on the mitogenic activity of fibroblast was studied to investigate its pharmic mechanism in the wound healing. In the experiments, using acetate buffer the tissues components were purified from the earthworm, which was crude extract. A group of proteins were obtained from the crude extract of the earthworm through ammonium sulfate precipitation, ion exchange chromatography and gel filtration chromatography. The effect of the group of proteins on the mitogenic activity of fibroblast was investigated with MTT method. It was illustrated that the group of proteins of mitogenic activity was on the first peak of Sephadex-G100, and presented three bands in SDS polyacrylamide gel electrophoresis. The range of their molecular weighs were 57 000, 72 000, 87 000. It can be drawn that there are some kind of proteins in the tissue components of the earthworm that has mitogenic activity of fibroblast.