黄芪穴位注射对运动性疲劳大鼠TH1/TH2细胞因子的影响

目的通过检测血清中白细胞介素-2(interleukin-2,IL-2)、γ干扰素(interferon-γ,IFN-γ)、IL-4,IL-10的含量,分析黄芪穴位注射对运动型疲劳大鼠TH1/TH2细胞因子的影响。方法健康SD雄性大鼠分为:正常对照组8只;疲劳模型空白干预组8只;疲劳模型黄芪注射组8只;疲劳模型0.9%氯化钠注射液注射组8只。疲劳模型黄芪注射组予以足三里穴位注射黄芪注射液0.1 mL。疲劳模型0.9%氯化钠注射液组予以足三里穴位注射0.1 mL 0.9%氯化钠注射液。每天游泳后称量体质量。末次游泳结束后,穴位注射1 h后,予以水合氯醛麻醉,心脏取血后,检测血清中IL-2、IFN-γ、IL-4、IL-10的含量。结果疲劳模型空白干预组血清IL-2及血清IFN-γ低于正常对照组;疲劳模型黄芪注射组的血清IL-2及血清IFN-γ高于疲劳模型空白干预组(P<0.01)。结论黄芪穴位注射可以减少运动性疲劳大鼠的体质量下降;可以调节运动性疲劳大鼠Th1/Th2细胞因子的平衡。

Effects of acupoint injection of Radix Astragali on Th1 and Th2 type cytokines in sport fatigue model rat

Objective To observe the effects of the acupoint injection of Radix Astragali Astragali on Th1 and Th2 type cytokines in Sport Fatigue Rats. Methods Totally 24 rats were divided into normal group, model group, acupoint injection of Radix Astragali group and acupoint injection of physiological saline group randomly, 8 rats in each group. The rats were injected with Radix Astragali and physiological saline in zusanli (ST36) acupoint respectively in acupoint injection of Radix Astragali group and acupoint injection of physiological saline group per-day after the swimming training. After the swimming training and the 10 days treatment, all the rats were injected with chloral hydrate for anesthesia. The blood was taken from each rat's heart and the contents of IL-2, IFN-γ, IL-4 and IL-10 in blood serum were examined. Results The serum levels of IL-2 and IFN-γ in model group decreased in comparison with those of normal group while The serum levels of IL-2 and IFN-γ in acupoint injection of Radix Astragali group increased obviously in comparison with those of model group(P<0.01). Conclusion Acupoint injection of Radix Astragali can prevent the rats from losing weight after swimming and can balance cytokines between type Th1 and Th2 cell.