EGF对人牙周膜细胞增生与ALP活性表达的影响

探讨表皮生长因子 (EGF)对人牙周膜细胞的增生与碱性磷酸酶 (ALP)活性表达的影响 ,为EGF应用于牙周组织的再生与修复提供实验依据。采用MTT和ALP活性检测法 ,观察不同质量浓度 ( 0 .1～ 1 0 μg/L)EGF第 2 4、4 8和 72h对体外培养的第 3代人牙周膜细胞 (PDLCs)的作用。与对照组比较 ,在 2 4～ 72h ,0 .1～ 5 μg/L的EGF可显著抑制PDLCs的增生 (P <0 .0 1～ 0 .0 5 ) ;1 0 μg/LEGF培养 4 8h对PDLCs有促进增生的作用 (P <0 .0 5 ) ,培养 72h有稳定增生的作用 (P >0 .0 5 )。 0 .1、0 .5 μg/LEGF可显著抑制PDLCs的ALP活性 (P <0 .0 1～ 0 .0 5 ) ;1～ 1 0 μg/LEGF在 2 4h可显著增强PDLCs的ALP活性 (P <0 .0 1～ 0 .0 5 ) ,在培养 4 8和 72h有稳定ALP活性的作用 (P >0 .0 5 )。提示在一定的作用时间内 ,1 0 μg/LEGF对人PDLCs的生长和分化可同时具有促进作用。

Effects of EGF on Proliferation and Alkaline Phosphatase Expression of Human Periodontal Ligament Cells

The aim of the study was to evaluate the biological effects of epidermal growth factor(EGF)on the proliferation and alkaline phosphatase(ALP)activity in cultured human periodontal ligament cells(PDLCs). Thiazoly blue(MTT)colorimetric assay and ALP activity analysis were used at different time points(24,48 and 72 h)to determine the proliferation and ALP activity of PDLCs after different doses of EGF were added into the culture medium. During 24～72 h, when compared with control group, 0.1～5.0μg/L EGF significantly inhibited the proliferation of PDLCs((P<0.01～0.05); 10 μg/L EGF significantly stimulated the proliferation of PDLCs at 48 h(P<0.05)and stabilized the proliferation of PDLCs at 72 h(P>0.05); 0.1～0.5μg/L EGF significantly decreased the ALP activity of PDLCs((P<0.01～0.05); 1～10μg/L EGF significantly increased the ALP activity of PDLCs((P<0.01～0.05)at 24 h and stabilized the ALP activity of PDLCs at 48 h and 72 h(P>0.05). These results suggest that 10 μg/L EGF may simultaneously promote the proliferation and differentiation of human PDLCs during a period of time.