| MT启动子控制的GFP基因在CHO细胞中的诱导表达 |
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| 引用本文： | 孙丽翠,祁雅慧,仲峰.MT启动子控制的GFP基因在CHO细胞中的诱导表达[J].首都医学院学报,2003,24(2):118-120. |
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| 作者姓名： | 孙丽翠 祁雅慧 仲峰 |
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| 作者单位： | [1]首都医科大学实验中心 [2]张家口老虎头煤矿职工医院 |
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| 摘 要： | 用羊金属硫蛋白(MT)启动子构建含绿色荧光蛋白(GFP)报告基因的真核表达载体,经脂质体介导在培养的中国仓鼠卵巢(CHO)细胞中进行诱导性表达。结果:构建的GFP基因表达载体能在CHO细胞中进行表达,用硫酸锌诱导可提高GFP的表达量。
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| 关 键 词： | MT启动子 诱导表达 GFP报告基因 |
| 收稿时间： | 2002-06-17 |
| 修稿时间： | 2002年6月17日 |
Inducible Expression of GFP Gene in CHO Cells under Control of MT Promoter |
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| Sun Licui,Qi Yahui,Zhong Feng.Inducible Expression of GFP Gene in CHO Cells under Control of MT Promoter[J].Journal of Capital University of Medical Sciences,2003,24(2):118-120. |
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| Authors: | Sun Licui Qi Yahui Zhong Feng |
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| Institution: | Sun Licui,Qi Yahui,Zhong Feng Department of Experiment Centre,Capital University of Medical Sciences Laohutou Colliery Hospital of Zhangjiakou |
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| Abstract: | Eukaryotic expression vector of GFP report gene under the control of sheep MT promoter was constructed. Result showed that the GFP gene in the vector could express itself inducibly in cultured CHO cells. The expression level of GFP could be increased by the inducement of ZnSO4. |
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| Keywords: | MT promoter inducible expression GFP report gene |
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