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APP17肽对卵巢去势大鼠海马神经元凋亡相关因子表达的影响
引用本文:谭宁,赵咏梅,等.APP17肽对卵巢去势大鼠海马神经元凋亡相关因子表达的影响[J].首都医学院学报,2001,22(4):296-299.
作者姓名:谭宁  赵咏梅
作者单位:首都医科大学宣武医院脑老化实验室(谭宁,盛树力),首都医科大学宣武医院脑老化实验室 (赵咏梅,赵志炜,姬志娟)
基金项目:科技部 973项目资助课题 (G2 0 0 0 0 5 70 10 )
摘    要:为研究卵巢去势大鼠海马神经元内凋亡相关因子Fas、Fas L、NFκB、c fos、c Jun的表达及APP1 7肽对这些因子表达的影响 ,将健康雌性Wistar大鼠行双侧卵巢切除手术造模 ,并用APP1 7肽治疗 ,分别用免疫组化方法观察Fas、Fas L、NFκB、c fos、c Jun的表达 ,用TUNEL方法检测神经元的凋亡。结果发现 :卵巢去势大鼠海马神经元Fas、Fas L、c fos、c Jun的表达增高 ,而NFκB的表达则减少 ;使用APP1 7肽治疗后 ,上述蛋白质的表达恢复到正常水平 ,TUNEL检测未发现凋亡神经元。提示卵巢去势大鼠发生了海马神经元的凋亡相关蛋白的改变 ,可能是神经元处于凋亡前状态 ;APP1 7肽可改善卵巢去势大鼠海马神经元内凋亡相关蛋白的表达 ,维持神经元的正常功能

关 键 词:卵巢去势  凋亡  APP17肽

Effect of APP17-mer Peptide on Expression of Apoptosis Related Proteins in Hippocampal Neurons of Ovariectomized Rats
Tan Ning,Zhao Yongmei,Zhao Zhiwei,Ji Zhijuan,Sheng Shuli Beijing Research Laboratory for Brain Aging,Xuanwu Hospital,Affiliate of Capital University of Medical Sciences.Effect of APP17-mer Peptide on Expression of Apoptosis Related Proteins in Hippocampal Neurons of Ovariectomized Rats[J].Journal of Capital University of Medical Sciences,2001,22(4):296-299.
Authors:Tan Ning  Zhao Yongmei  Zhao Zhiwei  Ji Zhijuan  Sheng Shuli Beijing Research Laboratory for Brain Aging  Xuanwu Hospital  Affiliate of Capital University of Medical Sciences
Abstract:To study the expressions of apoptosis related proteins Fas, Fas L, NFκB, c fos and c Jun in hippocampal neurons of ovariectomized rats, and the effect of APP17 mer peptide on these expressions, female Wistar rats were bilaterally ovariectomized (ovx), and were treated with APP17 mer peptide. Then they were studied by immunohistochemistry for Fas, Fas L, c fos, c Jun and NFκB, and were studied by TUNEL to observe the DNA fragments of neurons. The number of neurons stained positively by Fas, Fas L, c fos, c Jun antibodies in the brains of ovariectomized rats increased, and the number of neurons stained positively for NFκB was reduced. However, after being treated by the APP17 mer peptide, the expression of these apoptosis related proteins were normalized. There were no apoptotic neurons stained by TUNEL in the hippocampus. The result indicates that expression changes of apoptotic related protein in OVX rats. APP17 mer peptide treatment can normalize the expression of these proteins mentioned above. According to the research, the hippocampal neurons appear in a condition of pro apoptosis, but APP17 mer peptide is able to reverse the pro apoptosis situation of neurons.
Keywords:ovariectomy  apoptosis  APP17P
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