大鼠脑局部缺血再灌流后c-fos mRNA及c-Fos蛋白的表达

线栓法复制大鼠大脑中动脉缺血再灌流动物模型 ,于缺血 1h后分别再灌流 1h及 2h。原位杂交及免疫组化染色显示 ,再灌流 1h及 2h ,缺血侧额叶及顶叶皮质、海马各区以及齿状回c fosmRNA及c Fos蛋白表达均有不同程度诱导 ,于再灌流前电针“百会”及“风府”2穴 3 0min ,c fosmRNA和c Fos蛋白的诱导于再灌流 1h及 2h在缺血侧额叶、顶叶皮质 (P <0 .0 0 1 )及齿状回 (P <0 .0 1 )均显著增加 ;海马各区c Fos蛋白表达于再灌流 2h亦有显著增加(CA1与CA4:P <0 .0 5 ;CA2与CA3 :P <0 .0 1 )。该区c fosmRNA表达增加仅见于再灌流 1h的CA1及CA4区 (P <0 .0 5 )。HE染色示缺血区的神经元变性亦减轻。结果表明 :再灌流前给予电针处理使c fosmRNA及其产物c Fos蛋白表达增加 ,可能与电针对脑缺血再灌流动物模型的脑保护作用有关

Expression of c-fos mRNA and c-Fos Protein Following Focal Ischemia-Reperfusion in Rat and Effects of Electro-Acupunctur e

Reversible middle cerebral artery (MCA) occlusion in rats was made by intraluminal filament technique. After 1 h of ischemia, the filaments were withdrawn to allow reperfusion for 1 h and 2 h, respectively. In situ hybridyzation studies showed that after 1 h and 2 h of reperfusion, c fos mRNA was induced remarkably in ipsilateral ischemic neocortex (frontal, parietal), hippocampus CA1, CA2, CA3 and CA4 areas, and dentate gyrus. c Fos protein expression as demonstrated by immunohistochemical stain was also induced in all the brain areas mentioned above. With electroacupuncture at the points of "Baihui" and "Fengfu" for 30 min before reperfusion, both induction of c fos mRNA and c Fos protein were significantly enhanced in the cortex of frontal and parietal lobe ( P <0.001) and dentate gyrus ( P < 0.01) in ischemic hemisphere. In hippocampus, electro acupuncture increased the c Fos protein expression both after 1 h and 2 h of reperfusion significantly (CA1 and CA4: P <0.05; CA2 and CA3: P < 0.01), while marked increase of c fos mRNA occurred only after 1 h of reperfusion in CA1 and CA4 areas ( P <0.05). HE stain showed less neurol degeneration in ischemic area in rats that received electro acupuncture before reperfusion. The results showed that the treatment with electro acupuncture at the point of "Baihui" and "Fengfu" increased the expression of both c fos mRNA and c Fos protein after focal cerebral ischemia and reperfusion, which may play an important role in the brain protection of the animal model the authors used.